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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R292 (MIMI_R292) CSB-YP733037ADAZ
CSB-EP733037ADAZ
CSB-BP733037ADAZ
CSB-MP733037ADAZ
CSB-EP733037ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative collagenous domain-containing protein R238 (MIMI_R238) CSB-YP733038ADAZ
CSB-EP733038ADAZ
CSB-BP733038ADAZ
CSB-MP733038ADAZ
CSB-EP733038ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus DNA-directed RNA polymerase subunit 5 (MIMI_L235) CSB-YP733039ADAZ
CSB-EP733039ADAZ
CSB-BP733039ADAZ
CSB-MP733039ADAZ
CSB-EP733039ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L300 (MIMI_L300) CSB-YP733040ADAZ
CSB-EP733040ADAZ
CSB-BP733040ADAZ
CSB-MP733040ADAZ
CSB-EP733040ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus PP2C-like domain-containing protein R307 (MIMI_R307) CSB-YP733041ADAZ
CSB-EP733041ADAZ
CSB-BP733041ADAZ
CSB-MP733041ADAZ
CSB-EP733041ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R314 (MIMI_R314) CSB-YP733042ADAZ
CSB-EP733042ADAZ
CSB-BP733042ADAZ
CSB-MP733042ADAZ
CSB-EP733042ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ankyrin repeat protein R789 (MIMI_R789) CSB-YP733043ADAZ
CSB-EP733043ADAZ
CSB-BP733043ADAZ
CSB-MP733043ADAZ
CSB-EP733043ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Collagen-like protein 2 (MIMI_R196), partial CSB-YP733044ADAZ
CSB-EP733044ADAZ
CSB-BP733044ADAZ
CSB-MP733044ADAZ
CSB-EP733044ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative helicase L207/L206 (MIMI_L206, MIMI_L207), partial CSB-YP733045ADAZ
CSB-EP733045ADAZ
CSB-BP733045ADAZ
CSB-MP733045ADAZ
CSB-EP733045ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L211 (MIMI_L211), partial CSB-YP733046ADAZ1
CSB-EP733046ADAZ1
CSB-BP733046ADAZ1
CSB-MP733046ADAZ1
CSB-EP733046ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ariadne-like RING finger protein R811 (MIMI_R811), partial CSB-YP733047ADAZ
CSB-EP733047ADAZ
CSB-BP733047ADAZ
CSB-MP733047ADAZ
CSB-EP733047ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative ankyrin repeat protein L797 (MIMI_R797) CSB-YP733048ADAZ
CSB-EP733048ADAZ
CSB-BP733048ADAZ
CSB-MP733048ADAZ
CSB-EP733048ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R390 (MIMI_R390), partial CSB-YP733049ADAZ1
CSB-EP733049ADAZ1
CSB-BP733049ADAZ1
CSB-MP733049ADAZ1
CSB-EP733049ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Heat shock 70 kDa protein homolog (HSP70), partial CSB-YP733050ADAZ
CSB-EP733050ADAZ
CSB-BP733050ADAZ
CSB-MP733050ADAZ
CSB-EP733050ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L712 (MIMI_L712), partial CSB-YP733051ADAZ
CSB-EP733051ADAZ
CSB-BP733051ADAZ
CSB-MP733051ADAZ
CSB-EP733051ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R652 (MIMI_R652) CSB-YP733052ADAZ
CSB-EP733052ADAZ
CSB-BP733052ADAZ
CSB-MP733052ADAZ
CSB-EP733052ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R505 (MIMI_R505) CSB-YP733053ADAZ
CSB-EP733053ADAZ
CSB-BP733053ADAZ
CSB-MP733053ADAZ
CSB-EP733053ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L516 (MIMI_L516), partial CSB-YP733054ADAZ
CSB-EP733054ADAZ
CSB-BP733054ADAZ
CSB-MP733054ADAZ
CSB-EP733054ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R525 (MIMI_R525), partial CSB-YP733055ADAZ
CSB-EP733055ADAZ
CSB-BP733055ADAZ
CSB-MP733055ADAZ
CSB-EP733055ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative BTB/POZ domain-containing protein L520 (MIMI_L520), partial CSB-YP733056ADAZ
CSB-EP733056ADAZ
CSB-BP733056ADAZ
CSB-MP733056ADAZ
CSB-EP733056ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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