重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Haloarcula marismortui Phenylalanine--tRNA ligase beta subunit (pheT), partial | CSB-YP733117HTJ CSB-EP733117HTJ CSB-BP733117HTJ CSB-MP733117HTJ CSB-EP733117HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Cell division control protein 6 homolog 5 (cdc6-5) | CSB-YP733118HTJ CSB-EP733118HTJ CSB-BP733118HTJ CSB-MP733118HTJ CSB-EP733118HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui ATP phosphoribosyltransferase (hisG) | CSB-YP733119HTJ CSB-EP733119HTJ CSB-BP733119HTJ CSB-MP733119HTJ CSB-EP733119HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui 50S ribosomal protein L40e (rpl40e) | CSB-YP733120HTJ CSB-EP733120HTJ CSB-BP733120HTJ CSB-MP733120HTJ CSB-EP733120HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Uracil phosphoribosyltransferase (upp) | CSB-YP733121HTJ CSB-EP733121HTJ CSB-BP733121HTJ CSB-MP733121HTJ CSB-EP733121HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Putative HTH-type transcriptional regulatory protein rrnAC2519 (rrnAC2519) | CSB-YP733122HTJ CSB-EP733122HTJ CSB-BP733122HTJ CSB-MP733122HTJ CSB-EP733122HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Protein translation factor SUI1 homolog (sui1) | CSB-YP733123HTJ CSB-EP733123HTJ CSB-BP733123HTJ CSB-MP733123HTJ CSB-EP733123HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Elongation factor 2 (fusA), partial | CSB-YP733124HTJ CSB-EP733124HTJ CSB-BP733124HTJ CSB-MP733124HTJ CSB-EP733124HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Chemotaxis response regulator protein-glutamate methylesterase (cheB) | CSB-YP733125HTJ CSB-EP733125HTJ CSB-BP733125HTJ CSB-MP733125HTJ CSB-EP733125HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Translation initiation factor 5A (eif5a) | CSB-YP733126HTJ CSB-EP733126HTJ CSB-BP733126HTJ CSB-MP733126HTJ CSB-EP733126HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui 3-dehydroquinate dehydratase (aroD) | CSB-YP733127HTJ CSB-EP733127HTJ CSB-BP733127HTJ CSB-MP733127HTJ CSB-EP733127HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Proteasome subunit alpha 2 (psmA2) | CSB-YP733128HTJ CSB-EP733128HTJ CSB-BP733128HTJ CSB-MP733128HTJ CSB-EP733128HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Valine--tRNA ligase (valS), partial | CSB-YP733129HTJ CSB-EP733129HTJ CSB-BP733129HTJ CSB-MP733129HTJ CSB-EP733129HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) | CSB-YP733130HTJ CSB-EP733130HTJ CSB-BP733130HTJ CSB-MP733130HTJ CSB-EP733130HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Cell division control protein 6 homolog 2 (cdc6-2) | CSB-YP733131HTJ CSB-EP733131HTJ CSB-BP733131HTJ CSB-MP733131HTJ CSB-EP733131HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Ribonuclease P protein component 3 (rnp3) | CSB-YP733132HTJ CSB-EP733132HTJ CSB-BP733132HTJ CSB-MP733132HTJ CSB-EP733132HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui PyrE-like protein (pyrE2) | CSB-YP733133HTJ CSB-EP733133HTJ CSB-BP733133HTJ CSB-MP733133HTJ CSB-EP733133HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui D-tyrosyl-tRNA (Tyr) deacylase (dtdA) | CSB-YP733134HTJ CSB-EP733134HTJ CSB-BP733134HTJ CSB-MP733134HTJ CSB-EP733134HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui Glycerol kinase (glpK), partial | CSB-YP733135HTJ CSB-EP733135HTJ CSB-BP733135HTJ CSB-MP733135HTJ CSB-EP733135HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Haloarcula marismortui DNA ligase (lig), partial | CSB-YP733136HTJ CSB-EP733136HTJ CSB-BP733136HTJ CSB-MP733136HTJ CSB-EP733136HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022