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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Cytosol aminopeptidase (lap3), partial CSB-YP733397XBF
CSB-EP733397XBF
CSB-BP733397XBF
CSB-MP733397XBF
CSB-EP733397XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis PKHD domain-containing transmembrane protein C17orf101 homolog, partial CSB-YP733398XBF
CSB-EP733398XBF
CSB-BP733398XBF
CSB-MP733398XBF
CSB-EP733398XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ganglioside-induced differentiation-associated protein 2 CSB-YP733399XBE
CSB-EP733399XBE
CSB-BP733399XBE
CSB-MP733399XBE
CSB-EP733399XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 4b (chmp4b) CSB-YP733400XBE
CSB-EP733400XBE
CSB-BP733400XBE
CSB-MP733400XBE
CSB-EP733400XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NHP2-like protein 1 (nhp2l1) CSB-YP733401XBE
CSB-EP733401XBE
CSB-BP733401XBE
CSB-MP733401XBE
CSB-EP733401XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase MARCH8 (41341), partial CSB-YP733402XBE1
CSB-EP733402XBE1
CSB-BP733402XBE1
CSB-MP733402XBE1
CSB-EP733402XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis CCR4-NOT transcription complex subunit 6-like-B (cnot6l-b), partial CSB-YP733403XBE
CSB-EP733403XBE
CSB-BP733403XBE
CSB-MP733403XBE
CSB-EP733403XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Mitochondrial import inner membrane translocase subunit Tim23 (timm23), partial CSB-YP733404XBF1
CSB-EP733404XBF1
CSB-BP733404XBF1
CSB-MP733404XBF1
CSB-EP733404XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0602 protein C4orf47 homolog CSB-YP733405XBE
CSB-EP733405XBE
CSB-BP733405XBE
CSB-MP733405XBE
CSB-EP733405XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sphingomyelin phosphodiesterase 4 (smpd4), partial CSB-YP733406XBE
CSB-EP733406XBE
CSB-BP733406XBE
CSB-MP733406XBE
CSB-EP733406XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Testicular haploid expressed gene protein (Theg) CSB-YP733407RA
CSB-EP733407RA
CSB-BP733407RA
CSB-MP733407RA
CSB-EP733407RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat T-complex protein 11 homolog (Tcp11), partial CSB-YP733408RA1
CSB-EP733408RA1
CSB-BP733408RA1
CSB-MP733408RA1
CSB-EP733408RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 28S ribosomal protein S15, mitochondrial (Mrps15) CSB-YP733409RA
CSB-EP733409RA
CSB-BP733409RA
CSB-MP733409RA
CSB-EP733409RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat-containing protein 48 (Lrrc48) CSB-YP733410RA
CSB-EP733410RA
CSB-BP733410RA
CSB-MP733410RA
CSB-EP733410RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat F-box only protein 30 (Fbxo30) CSB-YP733411RA
CSB-EP733411RA
CSB-BP733411RA
CSB-MP733411RA
CSB-EP733411RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Alcohol dehydrogenase 6 (Adh6) CSB-YP733412RA
CSB-EP733412RA
CSB-BP733412RA
CSB-MP733412RA
CSB-EP733412RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat [Protein ADP-ribosylarginine] hydrolase-like protein 1 (Adprhl1) CSB-YP733413RA
CSB-EP733413RA
CSB-BP733413RA
CSB-MP733413RA
CSB-EP733413RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3) CSB-YP733414RA
CSB-EP733414RA
CSB-BP733414RA
CSB-MP733414RA
CSB-EP733414RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Armadillo repeat-containing X-linked protein 3 (Armcx3), partial CSB-YP733415RA
CSB-EP733415RA
CSB-BP733415RA
CSB-MP733415RA
CSB-EP733415RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat mTERF domain-containing protein 3, mitochondrial (Mterfd3) CSB-YP733416RA
CSB-EP733416RA
CSB-BP733416RA
CSB-MP733416RA
CSB-EP733416RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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