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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Mannheimia succiniciproducens Hydroxylamine reductase (hcp), partial CSB-YP734359MAAB
CSB-EP734359MAAB
CSB-BP734359MAAB
CSB-MP734359MAAB
CSB-EP734359MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP734360MAAB
CSB-EP734360MAAB
CSB-BP734360MAAB
CSB-MP734360MAAB
CSB-EP734360MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA1 (Val) (adenine (37)-N6)-methyltransferase (MS0203) CSB-YP734361MAAB
CSB-EP734361MAAB
CSB-BP734361MAAB
CSB-MP734361MAAB
CSB-EP734361MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 30S ribosomal protein S7 (rpsG) CSB-YP734362MAAB
CSB-EP734362MAAB
CSB-BP734362MAAB
CSB-MP734362MAAB
CSB-EP734362MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP734363MAAB
CSB-EP734363MAAB
CSB-BP734363MAAB
CSB-MP734363MAAB
CSB-EP734363MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP734364MAAB
CSB-EP734364MAAB
CSB-BP734364MAAB
CSB-MP734364MAAB
CSB-EP734364MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable serine/threonine-protein kinase WNK2 (WNK2), partial CSB-YP734365OFG
CSB-EP734365OFG
CSB-BP734365OFG
CSB-MP734365OFG
CSB-EP734365OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cystatin-A1 (cpiA) CSB-YP734366DKK
CSB-EP734366DKK
CSB-BP734366DKK
CSB-MP734366DKK
CSB-EP734366DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica mycoreovirus 1 Uncharacterized protein VP4 (S4), partial CSB-YP734367CFAT
CSB-EP734367CFAT
CSB-BP734367CFAT
CSB-MP734367CFAT
CSB-EP734367CFAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Protein yippee-like 5 (YPEL5) CSB-YP734368DSU
CSB-EP734368DSU
CSB-BP734368DSU
CSB-MP734368DSU
CSB-EP734368DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Glycoprotein N (gN), partial CSB-YP734369VAP1
CSB-EP734369VAP1
CSB-BP734369VAP1
CSB-MP734369VAP1
CSB-EP734369VAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP734370BUI
CSB-EP734370BUI
CSB-BP734370BUI
CSB-MP734370BUI
CSB-EP734370BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP734371BUI
CSB-EP734371BUI
CSB-BP734371BUI
CSB-MP734371BUI
CSB-EP734371BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carnation ringspot virus RNA-directed RNA polymerase (ORF1), partial CSB-YP734372CGAB
CSB-EP734372CGAB
CSB-BP734372CGAB
CSB-MP734372CGAB
CSB-EP734372CGAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP734373BUI
CSB-EP734373BUI
CSB-BP734373BUI
CSB-MP734373BUI
CSB-EP734373BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S16 (rpsP) CSB-YP734374BUI
CSB-EP734374BUI
CSB-BP734374BUI
CSB-MP734374BUI
CSB-EP734374BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP734375BUI
CSB-EP734375BUI
CSB-BP734375BUI
CSB-MP734375BUI
CSB-EP734375BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine--tRNA ligase (metG), partial CSB-YP734376BUI
CSB-EP734376BUI
CSB-BP734376BUI
CSB-MP734376BUI
CSB-EP734376BUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Replication protein 1a (ORF1a), partial CSB-YP734377CFAU
CSB-EP734377CFAU
CSB-BP734377CFAU
CSB-MP734377CFAU
CSB-EP734377CFAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP734378CGAZ
CSB-EP734378CGAZ
CSB-BP734378CGAZ
CSB-MP734378CGAZ
CSB-EP734378CGAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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