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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus licheniformis Bifunctional protein GlmU (glmU) CSB-YP734299BQU
CSB-EP734299BQU
CSB-BP734299BQU
CSB-MP734299BQU
CSB-EP734299BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Serine--tRNA ligase (serS) CSB-YP734300BQU
CSB-EP734300BQU
CSB-BP734300BQU
CSB-MP734300BQU
CSB-EP734300BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP734301MAAB
CSB-EP734301MAAB
CSB-BP734301MAAB
CSB-MP734301MAAB
CSB-EP734301MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens L-rhamnose-proton symporter (rhaT), partial CSB-YP734302MAAB
CSB-EP734302MAAB
CSB-BP734302MAAB
CSB-MP734302MAAB
CSB-EP734302MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Periplasmic nitrate reductase (napA), partial CSB-YP734303MAAB
CSB-EP734303MAAB
CSB-BP734303MAAB
CSB-MP734303MAAB
CSB-EP734303MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP734304MAAB
CSB-EP734304MAAB
CSB-BP734304MAAB
CSB-MP734304MAAB
CSB-EP734304MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Dihydroxy-acid dehydratase (ilvD), partial CSB-YP734305MAAB
CSB-EP734305MAAB
CSB-BP734305MAAB
CSB-MP734305MAAB
CSB-EP734305MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Large-conductance mechanosensitive channel (mscL), partial CSB-YP734306MAAB1
CSB-EP734306MAAB1
CSB-BP734306MAAB1
CSB-MP734306MAAB1
CSB-EP734306MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Maltose/maltodextrin import ATP-binding protein MalK (malK) CSB-YP734307MAAB
CSB-EP734307MAAB
CSB-BP734307MAAB
CSB-MP734307MAAB
CSB-EP734307MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Molybdenum cofactor guanylyltransferase (mobA) CSB-YP734308MAAB
CSB-EP734308MAAB
CSB-BP734308MAAB
CSB-MP734308MAAB
CSB-EP734308MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 50S ribosomal protein L16 (rplP) CSB-YP734309MAAB
CSB-EP734309MAAB
CSB-BP734309MAAB
CSB-MP734309MAAB
CSB-EP734309MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 30S ribosomal protein S4 (rpsD) CSB-YP734310MAAB
CSB-EP734310MAAB
CSB-BP734310MAAB
CSB-MP734310MAAB
CSB-EP734310MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Elongation factor Ts (tsf) CSB-YP734311MAAB
CSB-EP734311MAAB
CSB-BP734311MAAB
CSB-MP734311MAAB
CSB-EP734311MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP734312MAAB
CSB-EP734312MAAB
CSB-BP734312MAAB
CSB-MP734312MAAB
CSB-EP734312MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0352 protein MS1910 (MS1910) CSB-YP734313MAAB
CSB-EP734313MAAB
CSB-BP734313MAAB
CSB-MP734313MAAB
CSB-EP734313MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP734314MAAB
CSB-EP734314MAAB
CSB-BP734314MAAB
CSB-MP734314MAAB
CSB-EP734314MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glutamate 5-kinase (proB) CSB-YP734315MAAB
CSB-EP734315MAAB
CSB-BP734315MAAB
CSB-MP734315MAAB
CSB-EP734315MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Octanoyltransferase (lipB) CSB-YP734316MAAB
CSB-EP734316MAAB
CSB-BP734316MAAB
CSB-MP734316MAAB
CSB-EP734316MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Non-canonical purine NTP pyrophosphatase (MS1747) CSB-YP734317MAAB
CSB-EP734317MAAB
CSB-BP734317MAAB
CSB-MP734317MAAB
CSB-EP734317MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0042 nucleotide-binding protein MS1718 (MS1718) CSB-YP734318MAAB
CSB-EP734318MAAB
CSB-BP734318MAAB
CSB-MP734318MAAB
CSB-EP734318MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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