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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leifsonia xyli subsp. xyli 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP734820LAAC
CSB-EP734820LAAC
CSB-BP734820LAAC
CSB-MP734820LAAC
CSB-EP734820LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP734821LAAC
CSB-EP734821LAAC
CSB-BP734821LAAC
CSB-MP734821LAAC
CSB-EP734821LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli UvrABC system protein C (uvrC) CSB-YP734822
CSB-EP734822
CSB-BP734822
CSB-MP734822
CSB-EP734822-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP734823LAAC
CSB-EP734823LAAC
CSB-BP734823LAAC
CSB-MP734823LAAC
CSB-EP734823LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli N utilization substance protein B homolog (nusB) CSB-YP734824LAAC
CSB-EP734824LAAC
CSB-BP734824LAAC
CSB-MP734824LAAC
CSB-EP734824LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP734825LAAC
CSB-EP734825LAAC
CSB-BP734825LAAC
CSB-MP734825LAAC
CSB-EP734825LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP734826LAAC
CSB-EP734826LAAC
CSB-BP734826LAAC
CSB-MP734826LAAC
CSB-EP734826LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP734827LAAC
CSB-EP734827LAAC
CSB-BP734827LAAC
CSB-MP734827LAAC
CSB-EP734827LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli UPF0182 protein Lxx09300 (Lxx09300), partial CSB-YP734828LAAC
CSB-EP734828LAAC
CSB-BP734828LAAC
CSB-MP734828LAAC
CSB-EP734828LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli GTPase obg (obg), partial CSB-YP734829LAAC
CSB-EP734829LAAC
CSB-BP734829LAAC
CSB-MP734829LAAC
CSB-EP734829LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Trigger factor (tig) CSB-YP734830LAAC
CSB-EP734830LAAC
CSB-BP734830LAAC
CSB-MP734830LAAC
CSB-EP734830LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli ATP synthase subunit b (atpF), partial CSB-YP734831LAAC1
CSB-EP734831LAAC1
CSB-BP734831LAAC1
CSB-MP734831LAAC1
CSB-EP734831LAAC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP734832LAAC
CSB-EP734832LAAC
CSB-BP734832LAAC
CSB-MP734832LAAC
CSB-EP734832LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Thymidylate kinase (tmk) CSB-YP734833LAAC
CSB-EP734833LAAC
CSB-BP734833LAAC
CSB-MP734833LAAC
CSB-EP734833LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP734834LAAC
CSB-EP734834LAAC
CSB-BP734834LAAC
CSB-MP734834LAAC
CSB-EP734834LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Protein RecA (recA) CSB-YP734835DAAA
CSB-EP734835DAAA
CSB-BP734835DAAA
CSB-MP734835DAAA
CSB-EP734835DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila tRNA (Ile)-lysidine synthase (tilS) CSB-YP734836DAAA
CSB-EP734836DAAA
CSB-BP734836DAAA
CSB-MP734836DAAA
CSB-EP734836DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP734837DAAA
CSB-EP734837DAAA
CSB-BP734837DAAA
CSB-MP734837DAAA
CSB-EP734837DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Peptide chain release factor 3 (prfC), partial CSB-YP734838DAAA
CSB-EP734838DAAA
CSB-BP734838DAAA
CSB-MP734838DAAA
CSB-EP734838DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphopantetheine adenylyltransferase (coaD) CSB-YP734839DAAA
CSB-EP734839DAAA
CSB-BP734839DAAA
CSB-MP734839DAAA
CSB-EP734839DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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