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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Idiomarina loihiensis 50S ribosomal protein L1 (rplA) CSB-YP735597IAAA
CSB-EP735597IAAA
CSB-BP735597IAAA
CSB-MP735597IAAA
CSB-EP735597IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Catalase-peroxidase 2 (katG2), partial CSB-YP735598IAAA
CSB-EP735598IAAA
CSB-BP735598IAAA
CSB-MP735598IAAA
CSB-EP735598IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP735599IAAA
CSB-EP735599IAAA
CSB-BP735599IAAA
CSB-MP735599IAAA
CSB-EP735599IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis UPF0133 protein IL1848 (IL1848) CSB-YP735600IAAA
CSB-EP735600IAAA
CSB-BP735600IAAA
CSB-MP735600IAAA
CSB-EP735600IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Glucose-6-phosphate isomerase (pgi) CSB-YP735601IAAA
CSB-EP735601IAAA
CSB-BP735601IAAA
CSB-MP735601IAAA
CSB-EP735601IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP735602IAAA
CSB-EP735602IAAA
CSB-BP735602IAAA
CSB-MP735602IAAA
CSB-EP735602IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP735603IAAA
CSB-EP735603IAAA
CSB-BP735603IAAA
CSB-MP735603IAAA
CSB-EP735603IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP735604IAAA
CSB-EP735604IAAA
CSB-BP735604IAAA
CSB-MP735604IAAA
CSB-EP735604IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP735605IAAA
CSB-EP735605IAAA
CSB-BP735605IAAA
CSB-MP735605IAAA
CSB-EP735605IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Trigger factor (tig) CSB-YP735606IAAA
CSB-EP735606IAAA
CSB-BP735606IAAA
CSB-MP735606IAAA
CSB-EP735606IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 30S ribosomal protein S11 (rpsK) CSB-YP735607IAAA
CSB-EP735607IAAA
CSB-BP735607IAAA
CSB-MP735607IAAA
CSB-EP735607IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L15 (rplO) CSB-YP735608IAAA
CSB-EP735608IAAA
CSB-BP735608IAAA
CSB-MP735608IAAA
CSB-EP735608IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 30S ribosomal protein S14 (rpsN) CSB-YP735609IAAA
CSB-EP735609IAAA
CSB-BP735609IAAA
CSB-MP735609IAAA
CSB-EP735609IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L23 (rplW) CSB-YP735610IAAA
CSB-EP735610IAAA
CSB-BP735610IAAA
CSB-MP735610IAAA
CSB-EP735610IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L9 (rplI) CSB-YP735611IAAA
CSB-EP735611IAAA
CSB-BP735611IAAA
CSB-MP735611IAAA
CSB-EP735611IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP735612IAAA
CSB-EP735612IAAA
CSB-BP735612IAAA
CSB-MP735612IAAA
CSB-EP735612IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Probable Fe (2+)-trafficking protein (IL1984) CSB-YP735613IAAA
CSB-EP735613IAAA
CSB-BP735613IAAA
CSB-MP735613IAAA
CSB-EP735613IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Histidine--tRNA ligase (hisS) CSB-YP735614IAAA
CSB-EP735614IAAA
CSB-BP735614IAAA
CSB-MP735614IAAA
CSB-EP735614IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Lipoyl synthase (lipA) CSB-YP735615IAAA
CSB-EP735615IAAA
CSB-BP735615IAAA
CSB-MP735615IAAA
CSB-EP735615IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP735616IAAA
CSB-EP735616IAAA
CSB-BP735616IAAA
CSB-MP735616IAAA
CSB-EP735616IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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