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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacteroides fragilis Histidinol dehydrogenase (hisD) CSB-YP737412BDQ
CSB-EP737412BDQ
CSB-BP737412BDQ
CSB-MP737412BDQ
CSB-EP737412BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP737413BDQ
CSB-EP737413BDQ
CSB-BP737413BDQ
CSB-MP737413BDQ
CSB-EP737413BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis LL-diaminopimelate aminotransferase (dapL) CSB-YP737414BDQ
CSB-EP737414BDQ
CSB-BP737414BDQ
CSB-MP737414BDQ
CSB-EP737414BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Elongation factor 4 (lepA), partial CSB-YP737415BDQ
CSB-EP737415BDQ
CSB-BP737415BDQ
CSB-MP737415BDQ
CSB-EP737415BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis 50S ribosomal protein L28 (rpmB) CSB-YP737416BDQ
CSB-EP737416BDQ
CSB-BP737416BDQ
CSB-MP737416BDQ
CSB-EP737416BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP737417BDQ
CSB-EP737417BDQ
CSB-BP737417BDQ
CSB-MP737417BDQ
CSB-EP737417BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis tRNA1 (Val) (adenine (37)-N6)-methyltransferase (BF2305) CSB-YP737418BDQ
CSB-EP737418BDQ
CSB-BP737418BDQ
CSB-MP737418BDQ
CSB-EP737418BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP737419BDQ
CSB-EP737419BDQ
CSB-BP737419BDQ
CSB-MP737419BDQ
CSB-EP737419BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis ATP synthase gamma chain (atpG) CSB-YP737420BDQ
CSB-EP737420BDQ
CSB-BP737420BDQ
CSB-MP737420BDQ
CSB-EP737420BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis ATP synthase subunit c (atpE), partial CSB-YP737421BDQ1
CSB-EP737421BDQ1
CSB-BP737421BDQ1
CSB-MP737421BDQ1
CSB-EP737421BDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Threonine--tRNA ligase (thrS), partial CSB-YP737422BDQ
CSB-EP737422BDQ
CSB-BP737422BDQ
CSB-MP737422BDQ
CSB-EP737422BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis 7-cyano-7-deazaguanine synthase (queC) CSB-YP737423BDQ
CSB-EP737423BDQ
CSB-BP737423BDQ
CSB-MP737423BDQ
CSB-EP737423BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Peptidyl-tRNA hydrolase (pth) CSB-YP737424BDQ
CSB-EP737424BDQ
CSB-BP737424BDQ
CSB-MP737424BDQ
CSB-EP737424BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Type III pantothenate kinase (coaX) CSB-YP737425BDQ
CSB-EP737425BDQ
CSB-BP737425BDQ
CSB-MP737425BDQ
CSB-EP737425BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Ribonuclease Z (rnz) CSB-YP737426BDQ
CSB-EP737426BDQ
CSB-BP737426BDQ
CSB-MP737426BDQ
CSB-EP737426BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP737427BDQ
CSB-EP737427BDQ
CSB-BP737427BDQ
CSB-MP737427BDQ
CSB-EP737427BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Glycosyl hydrolase family 109 protein 2 (BF0955) CSB-YP737428BDQ
CSB-EP737428BDQ
CSB-BP737428BDQ
CSB-MP737428BDQ
CSB-EP737428BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP737429BDQ
CSB-EP737429BDQ
CSB-BP737429BDQ
CSB-MP737429BDQ
CSB-EP737429BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP737430BDQ
CSB-EP737430BDQ
CSB-BP737430BDQ
CSB-MP737430BDQ
CSB-EP737430BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Thymidine kinase (tdk) CSB-YP737431BDQ
CSB-EP737431BDQ
CSB-BP737431BDQ
CSB-MP737431BDQ
CSB-EP737431BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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