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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei UPF0042 nucleotide-binding protein BMA3112 (BMA3112) CSB-YP737111BNS
CSB-EP737111BNS
CSB-BP737111BNS
CSB-MP737111BNS
CSB-EP737111BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP synthase gamma chain (atpG) CSB-YP737112BNS
CSB-EP737112BNS
CSB-BP737112BNS
CSB-MP737112BNS
CSB-EP737112BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP737113BNS
CSB-EP737113BNS
CSB-BP737113BNS
CSB-MP737113BNS
CSB-EP737113BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP737114BNS
CSB-EP737114BNS
CSB-BP737114BNS
CSB-MP737114BNS
CSB-EP737114BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-dehydroquinate synthase (aroB) CSB-YP737115BNS
CSB-EP737115BNS
CSB-BP737115BNS
CSB-MP737115BNS
CSB-EP737115BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Histidinol dehydrogenase (hisD) CSB-YP737116BNS
CSB-EP737116BNS
CSB-BP737116BNS
CSB-MP737116BNS
CSB-EP737116BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP737117BNS
CSB-EP737117BNS
CSB-BP737117BNS
CSB-MP737117BNS
CSB-EP737117BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L11 (rplK) CSB-YP737118BNS
CSB-EP737118BNS
CSB-BP737118BNS
CSB-MP737118BNS
CSB-EP737118BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L4 (rplD) CSB-YP737119BNS
CSB-EP737119BNS
CSB-BP737119BNS
CSB-MP737119BNS
CSB-EP737119BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L5 (rplE) CSB-YP737120BNS
CSB-EP737120BNS
CSB-BP737120BNS
CSB-MP737120BNS
CSB-EP737120BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L6 (rplF) CSB-YP737121BNS
CSB-EP737121BNS
CSB-BP737121BNS
CSB-MP737121BNS
CSB-EP737121BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L17 (rplQ) CSB-YP737122BNS
CSB-EP737122BNS
CSB-BP737122BNS
CSB-MP737122BNS
CSB-EP737122BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP737123BNS
CSB-EP737123BNS
CSB-BP737123BNS
CSB-MP737123BNS
CSB-EP737123BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei GTPase obg (obg) CSB-YP737124BNS
CSB-EP737124BNS
CSB-BP737124BNS
CSB-MP737124BNS
CSB-EP737124BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydrodipicolinate reductase (dapB) CSB-YP737125BNS
CSB-EP737125BNS
CSB-BP737125BNS
CSB-MP737125BNS
CSB-EP737125BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP737126BNS
CSB-EP737126BNS
CSB-BP737126BNS
CSB-MP737126BNS
CSB-EP737126BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP737127BNS
CSB-EP737127BNS
CSB-BP737127BNS
CSB-MP737127BNS
CSB-EP737127BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaK (dnaK), partial CSB-YP737128BNS
CSB-EP737128BNS
CSB-BP737128BNS
CSB-MP737128BNS
CSB-EP737128BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP737129BNS
CSB-EP737129BNS
CSB-BP737129BNS
CSB-MP737129BNS
CSB-EP737129BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Elongation factor G 1 (fusA1), partial CSB-YP737130BNS
CSB-EP737130BNS
CSB-BP737130BNS
CSB-MP737130BNS
CSB-EP737130BNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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