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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Antiholin-like protein LrgA (lrgA), partial CSB-YP737171BAAD1
CSB-EP737171BAAD1
CSB-BP737171BAAD1
CSB-MP737171BAAD1
CSB-EP737171BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0741 protein BCE33L5081 (BCE33L5081) CSB-YP737172BAAD
CSB-EP737172BAAD
CSB-BP737172BAAD
CSB-MP737172BAAD
CSB-EP737172BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Serine hydroxymethyltransferase (glyA) CSB-YP737173BAAD
CSB-EP737173BAAD
CSB-BP737173BAAD
CSB-MP737173BAAD
CSB-EP737173BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Proto-oncogene tyrosine-protein kinase ROS (Ros1), partial CSB-YP737174RA
CSB-EP737174RA
CSB-BP737174RA
CSB-MP737174RA
CSB-EP737174RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tumor necrosis factor receptor superfamily member 6 (Fas), partial CSB-YP737175RA
CSB-EP737175RA
CSB-BP737175RA
CSB-MP737175RA
CSB-EP737175RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative sporulation transcription regulator WhiA (whiA) CSB-YP737176BAAD
CSB-EP737176BAAD
CSB-BP737176BAAD
CSB-MP737176BAAD
CSB-EP737176BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Low affinity immunoglobulin gamma Fc region receptor II (Fcgr2), partial CSB-YP737177RA
CSB-EP737177RA
CSB-BP737177RA
CSB-MP737177RA
CSB-EP737177RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Forkhead box protein D3 (Foxd3) CSB-YP737178RA
CSB-BP737178RA
CSB-MP737178RA
CSB-EP737178RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Forkhead box protein E3 (Foxe3) CSB-YP737179RA
CSB-EP737179RA
CSB-BP737179RA
CSB-MP737179RA
CSB-EP737179RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cytoplasmic aconitate hydratase (Aco1), partial CSB-YP737180RA
CSB-EP737180RA
CSB-BP737180RA
CSB-MP737180RA
CSB-EP737180RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus L-lactate dehydrogenase 2 (ldh2) CSB-YP737181BAAD
CSB-EP737181BAAD
CSB-BP737181BAAD
CSB-MP737181BAAD
CSB-EP737181BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Pleckstrin homology-like domain family B member 1 (Phldb1), partial CSB-YP737182RA
CSB-EP737182RA
CSB-BP737182RA
CSB-MP737182RA
CSB-EP737182RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (Plod1) CSB-YP737183RA
CSB-EP737183RA
CSB-BP737183RA
CSB-MP737183RA
CSB-EP737183RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (Ndufa5) CSB-YP737184RA
CSB-EP737184RA
CSB-BP737184RA
CSB-MP737184RA
CSB-EP737184RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat P2Y purinoceptor 6 (P2ry6), partial CSB-YP737185RA1
CSB-EP737185RA1
CSB-BP737185RA1
CSB-MP737185RA1
CSB-EP737185RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium/potassium-transporting ATPase subunit beta-3 (Atp1b3), partial CSB-YP737186RA
CSB-EP737186RA
CSB-BP737186RA
CSB-MP737186RA
CSB-EP737186RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Large-conductance mechanosensitive channel (mscL), partial CSB-YP737187BAAD1
CSB-EP737187BAAD1
CSB-BP737187BAAD1
CSB-MP737187BAAD1
CSB-EP737187BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dephospho-CoA kinase (coaE) CSB-YP737188BAAD
CSB-EP737188BAAD
CSB-BP737188BAAD
CSB-MP737188BAAD
CSB-EP737188BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein I (sspI) CSB-YP737189BAAD
CSB-EP737189BAAD
CSB-BP737189BAAD
CSB-MP737189BAAD
CSB-EP737189BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heme-degrading monooxygenase isdG (isdG) CSB-YP737190BAAD
CSB-EP737190BAAD
CSB-BP737190BAAD
CSB-MP737190BAAD
CSB-EP737190BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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