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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Lipoma HMGIC fusion partner-like 3 protein (lhfpl3), partial CSB-YP738553DIL1
CSB-EP738553DIL1
CSB-BP738553DIL1
CSB-MP738553DIL1
CSB-EP738553DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Aspartoacylase-2A (acy3.1) CSB-YP738554DIL
CSB-EP738554DIL
CSB-BP738554DIL
CSB-MP738554DIL
CSB-EP738554DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Voltage-gated hydrogen channel 1 (hvcn1), partial CSB-YP738555DIL1
CSB-EP738555DIL1
CSB-BP738555DIL1
CSB-MP738555DIL1
CSB-EP738555DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lymphokine-activated killer T-cell-originated protein kinase homolog (pbk) CSB-YP738556DIL
CSB-EP738556DIL
CSB-BP738556DIL
CSB-MP738556DIL
CSB-EP738556DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Nucleoside diphosphate kinase 6 (nme6) CSB-YP738557DIL
CSB-EP738557DIL
CSB-BP738557DIL
CSB-MP738557DIL
CSB-EP738557DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C20orf112 homolog CSB-YP738558MO
CSB-EP738558MO
CSB-BP738558MO
CSB-MP738558MO
CSB-EP738558MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Homeobox protein meis3 (meis3) CSB-YP738559XBF
CSB-EP738559XBF
CSB-BP738559XBF
CSB-MP738559XBF
CSB-EP738559XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ubiquitin carboxyl-terminal hydrolase 10 (usp10), partial CSB-YP738560XBF
CSB-EP738560XBF
CSB-BP738560XBF
CSB-MP738560XBF
CSB-EP738560XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Histone chaperone asf1b (asf1b) CSB-YP738561XBF
CSB-EP738561XBF
CSB-BP738561XBF
CSB-MP738561XBF
CSB-EP738561XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Testin (tes) CSB-YP738562XBF
CSB-EP738562XBF
CSB-BP738562XBF
CSB-MP738562XBF
CSB-EP738562XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis RNA-binding protein 38 (rbm38) CSB-YP738563XBF
CSB-EP738563XBF
CSB-BP738563XBF
CSB-MP738563XBF
CSB-EP738563XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform (ppp2r2d) CSB-YP738564XBF
CSB-EP738564XBF
CSB-BP738564XBF
CSB-MP738564XBF
CSB-EP738564XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein phosphatase 2A regulatory subunit B' subunit gamma (ppp2r3c) CSB-YP738565XBF
CSB-EP738565XBF
CSB-BP738565XBF
CSB-MP738565XBF
CSB-EP738565XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0552 protein C15orf38 homolog CSB-YP738566XBF
CSB-EP738566XBF
CSB-BP738566XBF
CSB-MP738566XBF
CSB-EP738566XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein argonaute-2 (eif2c2), partial CSB-YP738567XBF
CSB-EP738567XBF
CSB-BP738567XBF
CSB-MP738567XBF
CSB-EP738567XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis BRISC and BRCA1-A complex member 1 (babam1) CSB-YP738568XBE
CSB-EP738568XBE
CSB-BP738568XBE
CSB-MP738568XBE
CSB-EP738568XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coiled-coil domain-containing protein 130 (ccdc130) CSB-YP738569XBE
CSB-EP738569XBE
CSB-BP738569XBE
CSB-MP738569XBE
CSB-EP738569XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis POU domain, class 3, transcription factor 4-B (pou3f4-b) CSB-YP738570XBE
CSB-EP738570XBE
CSB-BP738570XBE
CSB-MP738570XBE
CSB-EP738570XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Small ubiquitin-related modifier 3 (sumo3) CSB-YP738571XBF
CSB-EP738571XBF
CSB-BP738571XBF
CSB-MP738571XBF
CSB-EP738571XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WASH complex subunit CCDC53 (ccdc53) CSB-YP738572XBE
CSB-EP738572XBE
CSB-BP738572XBE
CSB-MP738572XBE
CSB-EP738572XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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