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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Acinetobacter sp. Ketol-acid reductoisomerase (ilvC) CSB-YP739375AWW
CSB-EP739375AWW
CSB-BP739375AWW
CSB-MP739375AWW
CSB-EP739375AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Threonine--tRNA ligase (thrS), partial CSB-YP739376AWW
CSB-EP739376AWW
CSB-BP739376AWW
CSB-MP739376AWW
CSB-EP739376AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Valine--tRNA ligase (valS), partial CSB-YP739377AWW
CSB-EP739377AWW
CSB-BP739377AWW
CSB-MP739377AWW
CSB-EP739377AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Octanoyltransferase (lipB) CSB-YP739378AWW
CSB-EP739378AWW
CSB-BP739378AWW
CSB-MP739378AWW
CSB-EP739378AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP739379AWW
CSB-EP739379AWW
CSB-BP739379AWW
CSB-MP739379AWW
CSB-EP739379AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP739380AWW
CSB-EP739380AWW
CSB-BP739380AWW
CSB-MP739380AWW
CSB-EP739380AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Protein TolB (tolB) CSB-YP739381AWW
CSB-EP739381AWW
CSB-BP739381AWW
CSB-MP739381AWW
CSB-EP739381AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0271 protein ACIAD2520 (ACIAD2520) CSB-YP739382AWW
CSB-EP739382AWW
CSB-BP739382AWW
CSB-MP739382AWW
CSB-EP739382AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribosomal protein L11 methyltransferase (prmA) CSB-YP739383AWW
CSB-EP739383AWW
CSB-BP739383AWW
CSB-MP739383AWW
CSB-EP739383AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative phosphotransferase ACIAD2422 (ACIAD2422) CSB-YP739384AWW
CSB-EP739384AWW
CSB-BP739384AWW
CSB-MP739384AWW
CSB-EP739384AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP739385AWW
CSB-EP739385AWW
CSB-BP739385AWW
CSB-MP739385AWW
CSB-EP739385AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. [Protein-PII] uridylyltransferase (glnD), partial CSB-YP739386AWW
CSB-EP739386AWW
CSB-BP739386AWW
CSB-MP739386AWW
CSB-EP739386AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Chorismate synthase (aroC) CSB-YP739387AWW
CSB-EP739387AWW
CSB-BP739387AWW
CSB-MP739387AWW
CSB-EP739387AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Maf-like protein ACIAD1930 (ACIAD1930) CSB-YP739388AWW
CSB-EP739388AWW
CSB-BP739388AWW
CSB-MP739388AWW
CSB-EP739388AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 3-dehydroquinate dehydratase (aroQ) CSB-YP739389AWW
CSB-EP739389AWW
CSB-BP739389AWW
CSB-MP739389AWW
CSB-EP739389AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative regulator of ribonuclease activity (ACIAD1391) CSB-YP739390AWW
CSB-EP739390AWW
CSB-BP739390AWW
CSB-MP739390AWW
CSB-EP739390AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP739391AWW
CSB-EP739391AWW
CSB-BP739391AWW
CSB-MP739391AWW
CSB-EP739391AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Thiazole synthase (thiG) CSB-YP739392AWW
CSB-EP739392AWW
CSB-BP739392AWW
CSB-MP739392AWW
CSB-EP739392AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Alanine--tRNA ligase (alaS), partial CSB-YP739393AWW
CSB-EP739393AWW
CSB-BP739393AWW
CSB-MP739393AWW
CSB-EP739393AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP739394AWW
CSB-EP739394AWW
CSB-BP739394AWW
CSB-MP739394AWW
CSB-EP739394AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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