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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mouse G-protein coupled receptor 126 (Gpr126), partial CSB-YP739635MO
CSB-EP739635MO
CSB-BP739635MO
CSB-MP739635MO
CSB-EP739635MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Curculigo latifolia Curculin-2 CSB-YP739636CUT
CSB-EP739636CUT
CSB-BP739636CUT
CSB-MP739636CUT
CSB-EP739636CUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Membrane protein 0 (ORF0), partial CSB-YP739637VAP1
CSB-EP739637VAP1
CSB-BP739637VAP1
CSB-MP739637VAP1
CSB-EP739637VAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Methionyl-tRNA formyltransferase (fmt) CSB-YP739638AWW
CSB-EP739638AWW
CSB-BP739638AWW
CSB-MP739638AWW
CSB-EP739638AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0391 membrane protein ACIAD3602 (ACIAD3602), partial CSB-YP739639AWW
CSB-EP739639AWW
CSB-BP739639AWW
CSB-MP739639AWW
CSB-EP739639AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP739640AWW
CSB-EP739640AWW
CSB-BP739640AWW
CSB-MP739640AWW
CSB-EP739640AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP739641AWW
CSB-EP739641AWW
CSB-BP739641AWW
CSB-MP739641AWW
CSB-EP739641AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Probable potassium transport system protein kup (kup), partial CSB-YP739642AWW
CSB-EP739642AWW
CSB-BP739642AWW
CSB-MP739642AWW
CSB-EP739642AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Shikimate kinase (aroK) CSB-YP739643AWW
CSB-EP739643AWW
CSB-BP739643AWW
CSB-MP739643AWW
CSB-EP739643AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Ribosome maturation factor RimM (rimM) CSB-YP739644AWW
CSB-EP739644AWW
CSB-BP739644AWW
CSB-MP739644AWW
CSB-EP739644AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP739645AWW
CSB-EP739645AWW
CSB-BP739645AWW
CSB-MP739645AWW
CSB-EP739645AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L2 (rplB) CSB-YP739646AWW
CSB-EP739646AWW
CSB-BP739646AWW
CSB-MP739646AWW
CSB-EP739646AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L14 (rplN) CSB-YP739647AWW
CSB-EP739647AWW
CSB-BP739647AWW
CSB-MP739647AWW
CSB-EP739647AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L6 (rplF) CSB-YP739648AWW
CSB-EP739648AWW
CSB-BP739648AWW
CSB-MP739648AWW
CSB-EP739648AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP739649AWW
CSB-EP739649AWW
CSB-BP739649AWW
CSB-MP739649AWW
CSB-EP739649AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0234 protein ACIAD3137 (ACIAD3137) CSB-YP739650AWW
CSB-EP739650AWW
CSB-BP739650AWW
CSB-MP739650AWW
CSB-EP739650AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP739651AWW
CSB-EP739651AWW
CSB-BP739651AWW
CSB-MP739651AWW
CSB-EP739651AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L35 (rpmI) CSB-YP739652AWW
CSB-EP739652AWW
CSB-BP739652AWW
CSB-MP739652AWW
CSB-EP739652AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0145 protein ACIAD2946 (ACIAD2946) CSB-YP739653AWW
CSB-EP739653AWW
CSB-BP739653AWW
CSB-MP739653AWW
CSB-EP739653AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Glutamyl-tRNA reductase (hemA) CSB-YP739654AWW
CSB-EP739654AWW
CSB-BP739654AWW
CSB-MP739654AWW
CSB-EP739654AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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