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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana HVA22-like protein k (HVA22K) CSB-YP740635DOA
CSB-EP740635DOA
CSB-BP740635DOA
CSB-MP740635DOA
CSB-EP740635DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CLAVATA3/ESR (CLE)-related protein 13 (CLE13) CSB-YP740636DOA
CSB-EP740636DOA
CSB-BP740636DOA
CSB-MP740636DOA
CSB-EP740636DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein FANTASTIC FOUR 3 (FAF3) CSB-YP740637DOA
CSB-EP740637DOA
CSB-BP740637DOA
CSB-MP740637DOA
CSB-EP740637DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein phosphatase Slingshot (ssh), partial CSB-YP740638DLU
CSB-EP740638DLU
CSB-BP740638DLU
CSB-MP740638DLU
CSB-EP740638DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g43822 (At5g43822) CSB-YP740639DOA
CSB-EP740639DOA
CSB-BP740639DOA
CSB-MP740639DOA
CSB-EP740639DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tubby-like F-box protein 11 (TULP11) CSB-YP740640DOA
CSB-EP740640DOA
CSB-BP740640DOA
CSB-MP740640DOA
CSB-EP740640DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein DAMAGED DNA-BINDING 2 (DDB2), partial CSB-YP740641DOA
CSB-EP740641DOA
CSB-BP740641DOA
CSB-MP740641DOA
CSB-EP740641DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Bidirectional sugar transporter SWEET3 (SWEET3), partial CSB-YP740642DOA1
CSB-EP740642DOA1
CSB-BP740642DOA1
CSB-MP740642DOA1
CSB-EP740642DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADPH-dependent diflavin oxidoreductase 1 (ndor1), partial CSB-YP740643XBE
CSB-EP740643XBE
CSB-BP740643XBE
CSB-MP740643XBE
CSB-EP740643XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0667 protein C1orf55 homolog CSB-YP740644XBE
CSB-EP740644XBE
CSB-BP740644XBE
CSB-MP740644XBE
CSB-EP740644XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (agap1), partial CSB-YP740645XBE
CSB-EP740645XBE
CSB-BP740645XBE
CSB-MP740645XBE
CSB-EP740645XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein 238.3 (znf238.3), partial CSB-YP740646XBE
CSB-EP740646XBE
CSB-BP740646XBE
CSB-MP740646XBE
CSB-EP740646XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WD40 repeat-containing protein SMU1 (smu1), partial CSB-YP740647XBE
CSB-EP740647XBE
CSB-BP740647XBE
CSB-MP740647XBE
CSB-EP740647XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF149 (rnf149), partial CSB-YP740648XBE1
CSB-EP740648XBE1
CSB-BP740648XBE1
CSB-MP740648XBE1
CSB-EP740648XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UHRF1-binding protein 1-like (uhrf1bp1l), partial CSB-YP740649XBE
CSB-EP740649XBE
CSB-BP740649XBE
CSB-MP740649XBE
CSB-EP740649XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1) CSB-YP740650XBE
CSB-BP740650XBE
CSB-MP740650XBE
CSB-EP740650XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein FAM135A (Fam135a), partial CSB-YP740651MO
CSB-EP740651MO
CSB-BP740651MO
CSB-MP740651MO
CSB-EP740651MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein CXorf57 (CXorf57), partial CSB-YP740652HU
CSB-EP740652HU
CSB-BP740652HU
CSB-MP740652HU
CSB-EP740652HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse A-kinase anchor protein SPHKAP (Sphkap), partial CSB-YP740653MO
CSB-EP740653MO
CSB-BP740653MO
CSB-MP740653MO
CSB-EP740653MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytochrome P450 4F22 (CYP4F22) CSB-YP740654HU
CSB-EP740654HU
CSB-BP740654HU
CSB-MP740654HU
CSB-EP740654HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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