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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dihydroorotase (pyrC) CSB-YP752012SMU
CSB-EP752012SMU
CSB-BP752012SMU
CSB-MP752012SMU
CSB-EP752012SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP752013SMU
CSB-EP752013SMU
CSB-BP752013SMU
CSB-MP752013SMU
CSB-EP752013SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Xaa-Pro dipeptidase (pepQ) CSB-YP752014SXB
CSB-EP752014SXB
CSB-BP752014SXB
CSB-MP752014SXB
CSB-EP752014SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein AaeX (aaeX), partial CSB-YP752015SXB1
CSB-EP752015SXB1
CSB-BP752015SXB1
CSB-MP752015SXB1
CSB-EP752015SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium HTH-type transcriptional regulator iscR (iscR) CSB-YP752016SXB
CSB-EP752016SXB
CSB-BP752016SXB
CSB-MP752016SXB
CSB-EP752016SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP752017SXB
CSB-EP752017SXB
CSB-BP752017SXB
CSB-MP752017SXB
CSB-EP752017SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0387 membrane protein yohO (yohO), partial CSB-YP752018SXB
CSB-EP752018SXB
CSB-BP752018SXB
CSB-MP752018SXB
CSB-EP752018SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium OriC-binding nucleoid-associated protein (cnu) CSB-YP752019SXB
CSB-EP752019SXB
CSB-BP752019SXB
CSB-MP752019SXB
CSB-EP752019SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Sulfurtransferase TusE (tusE) CSB-YP752020SXB
CSB-EP752020SXB
CSB-BP752020SXB
CSB-MP752020SXB
CSB-EP752020SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA protection during starvation protein (dps) CSB-YP752021SXB
CSB-EP752021SXB
CSB-BP752021SXB
CSB-MP752021SXB
CSB-EP752021SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0325 protein yaeH (yaeH) CSB-YP752022SXB
CSB-EP752022SXB
CSB-BP752022SXB
CSB-MP752022SXB
CSB-EP752022SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Adenine deaminase 2 (ade2), partial CSB-YP752023AYS
CSB-EP752023AYS
CSB-BP752023AYS
CSB-MP752023AYS
CSB-EP752023AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Thymidine kinase (tdk) CSB-YP752024AYS
CSB-EP752024AYS
CSB-BP752024AYS
CSB-MP752024AYS
CSB-EP752024AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP752025AYS
CSB-EP752025AYS
CSB-BP752025AYS
CSB-MP752025AYS
CSB-EP752025AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium fabrum N-acyl homoserine lactonase AttM (attM) CSB-YP752026AYS
CSB-EP752026AYS
CSB-BP752026AYS
CSB-MP752026AYS
CSB-EP752026AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE62 (ppe62), partial CSB-YP752027MVZ
CSB-EP752027MVZ
CSB-BP752027MVZ
CSB-MP752027MVZ
CSB-EP752027MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CDP-diacylglycerol--inositol 3-phosphatidyltransferase (pgsA1), partial CSB-YP752028MVZ1
CSB-EP752028MVZ1
CSB-BP752028MVZ1
CSB-MP752028MVZ1
CSB-EP752028MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE38 (ppe38) CSB-YP752029MVZ
CSB-EP752029MVZ
CSB-BP752029MVZ
CSB-MP752029MVZ
CSB-EP752029MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE15 (ppe15) CSB-YP752030MVZ
CSB-EP752030MVZ
CSB-BP752030MVZ
CSB-MP752030MVZ
CSB-EP752030MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translocated intimin receptor Tir (tir), partial CSB-YP752031EOD
CSB-EP752031EOD
CSB-BP752031EOD
CSB-MP752031EOD
CSB-EP752031EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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