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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP752815EYQ
CSB-EP752815EYQ
CSB-BP752815EYQ
CSB-MP752815EYQ
CSB-EP752815EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP synthase subunit delta (atpH) CSB-YP752816EYQ
CSB-EP752816EYQ
CSB-BP752816EYQ
CSB-MP752816EYQ
CSB-EP752816EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribonuclease Z (rnz) CSB-YP752817EYQ
CSB-EP752817EYQ
CSB-BP752817EYQ
CSB-MP752817EYQ
CSB-EP752817EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP752818EYQ1
CSB-EP752818EYQ1
CSB-BP752818EYQ1
CSB-MP752818EYQ1
CSB-EP752818EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Apolipoprotein N-acyltransferase (lnt), partial CSB-YP752819EYQ1
CSB-EP752819EYQ1
CSB-BP752819EYQ1
CSB-MP752819EYQ1
CSB-EP752819EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L34 (rpmH) CSB-YP752820EYQ
CSB-EP752820EYQ
CSB-BP752820EYQ
CSB-MP752820EYQ
CSB-EP752820EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP752821EYQ
CSB-EP752821EYQ
CSB-BP752821EYQ
CSB-MP752821EYQ
CSB-EP752821EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Undecaprenyl pyrophosphate synthase (uppS) CSB-YP752822EYQ
CSB-EP752822EYQ
CSB-BP752822EYQ
CSB-MP752822EYQ
CSB-EP752822EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Urease accessory protein UreD (ureD) CSB-YP752823EYQ
CSB-EP752823EYQ
CSB-BP752823EYQ
CSB-MP752823EYQ
CSB-EP752823EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP752824EYQ
CSB-EP752824EYQ
CSB-BP752824EYQ
CSB-MP752824EYQ
CSB-EP752824EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glucose-6-phosphate isomerase (pgi), partial CSB-YP752825EYQ
CSB-EP752825EYQ
CSB-BP752825EYQ
CSB-MP752825EYQ
CSB-EP752825EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Triosephosphate isomerase (tpiA) CSB-YP752826EYQ
CSB-EP752826EYQ
CSB-BP752826EYQ
CSB-MP752826EYQ
CSB-EP752826EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP752827EYQ
CSB-EP752827EYQ
CSB-BP752827EYQ
CSB-MP752827EYQ
CSB-EP752827EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Phosphate import ATP-binding protein PstB (pstB) CSB-YP752828EYQ
CSB-EP752828EYQ
CSB-BP752828EYQ
CSB-MP752828EYQ
CSB-EP752828EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glycogen synthase (glgA) CSB-YP752829EYQ
CSB-EP752829EYQ
CSB-BP752829EYQ
CSB-MP752829EYQ
CSB-EP752829EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I reaction center subunit XII (psaM), partial CSB-YP752830EYQ
CSB-EP752830EYQ
CSB-BP752830EYQ
CSB-MP752830EYQ
CSB-EP752830EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Porphobilinogen deaminase (hemC) CSB-YP752831EYQ
CSB-EP752831EYQ
CSB-BP752831EYQ
CSB-MP752831EYQ
CSB-EP752831EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I reaction center subunit IX (psaJ), partial CSB-YP752832EYQ
CSB-EP752832EYQ
CSB-BP752832EYQ
CSB-MP752832EYQ
CSB-EP752832EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP752833EYQ
CSB-EP752833EYQ
CSB-BP752833EYQ
CSB-MP752833EYQ
CSB-EP752833EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Octanoyltransferase (lipB) CSB-YP752834EYQ
CSB-EP752834EYQ
CSB-BP752834EYQ
CSB-MP752834EYQ
CSB-EP752834EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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