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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chicken Collagen alpha-1 (VIII) chain (COL8A1), partial CSB-YP752113CH
CSB-EP752113CH
CSB-BP752113CH
CSB-MP752113CH
CSB-EP752113CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 39S ribosomal protein L14, mitochondrial (Mrpl14) CSB-YP752114RA
CSB-EP752114RA
CSB-BP752114RA
CSB-MP752114RA
CSB-EP752114RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase CSB-YP752115DUN
CSB-EP752115DUN
CSB-BP752115DUN
CSB-MP752115DUN
CSB-EP752115DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lolium perenne Major pollen allergen Lol p 11 (X103S) CSB-YP752117LQK
CSB-EP752117LQK
CSB-BP752117LQK
CSB-MP752117LQK
CSB-EP752117LQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Chymotrypsin-C (CTRC) CSB-YP752118BO
CSB-EP752118BO
CSB-BP752118BO
CSB-MP752118BO
CSB-EP752118BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa orientalis Orientotoxin-2 CSB-YP752119VBS
CSB-EP752119VBS
CSB-BP752119VBS
CSB-MP752119VBS
CSB-EP752119VBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sepia officinalis Histone H4 CSB-YP752120SYJ
CSB-EP752120SYJ
CSB-BP752120SYJ
CSB-MP752120SYJ
CSB-EP752120SYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bunodosoma caissarum Major neurotoxin BcIII CSB-YP752121BNJ
CSB-EP752121BNJ
CSB-BP752121BNJ
CSB-MP752121BNJ
CSB-EP752121BNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tulipa bakeri Chitinase 2 CSB-YP752122TCAP
CSB-EP752122TCAP
CSB-BP752122TCAP
CSB-MP752122TCAP
CSB-EP752122TCAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistocerca gregaria Endocuticle structural protein SgAbd-6 CSB-YP752123SXO
CSB-EP752123SXO
CSB-BP752123SXO
CSB-MP752123SXO
CSB-EP752123SXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fasciola hepatica Fatty acid-binding protein Fh15 CSB-YP752124FEI
CSB-EP752124FEI
CSB-BP752124FEI
CSB-MP752124FEI
CSB-EP752124FEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YBL113C (YBL113C), partial CSB-YP752125SVG
CSB-EP752125SVG
CSB-BP752125SVG
CSB-MP752125SVG
CSB-EP752125SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL21A (UL21A) CSB-YP752126HWV
CSB-EP752126HWV
CSB-BP752126HWV
CSB-MP752126HWV
CSB-EP752126HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat N-lysine methyltransferase SMYD2 (Smyd2) CSB-YP752127RA
CSB-EP752127RA
CSB-BP752127RA
CSB-MP752127RA
CSB-EP752127RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Taste receptor type 2 member 110 (Tas2r110), partial CSB-YP752128MO1
CSB-EP752128MO1
CSB-BP752128MO1
CSB-MP752128MO1
CSB-EP752128MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Taste receptor type 2 member 114 (Tas2r114), partial CSB-YP752129MO1
CSB-EP752129MO1
CSB-BP752129MO1
CSB-MP752129MO1
CSB-EP752129MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Otopetrin-1 (Otop1), partial CSB-YP752130RA
CSB-EP752130RA
CSB-BP752130RA
CSB-MP752130RA
CSB-EP752130RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Abhydrolase domain-containing protein FAM108B1 (Fam108b1) CSB-YP752131MO
CSB-EP752131MO
CSB-BP752131MO
CSB-MP752131MO
CSB-EP752131MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 10 kDa chaperonin 2 (groS2) CSB-YP752132VCQ
CSB-EP752132VCQ
CSB-BP752132VCQ
CSB-MP752132VCQ
CSB-EP752132VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Deoxyguanosinetriphosphate triphosphohydrolase-like protein (VV1112) CSB-YP752133VCQ
CSB-EP752133VCQ
CSB-BP752133VCQ
CSB-MP752133VCQ
CSB-EP752133VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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