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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP752715SSL
CSB-EP752715SSL
CSB-BP752715SSL
CSB-MP752715SSL
CSB-EP752715SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Shikimate kinase (aroK) CSB-YP752716SSL
CSB-EP752716SSL
CSB-BP752716SSL
CSB-MP752716SSL
CSB-EP752716SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S10 (rpsJ) CSB-YP752717SSL
CSB-EP752717SSL
CSB-BP752717SSL
CSB-MP752717SSL
CSB-EP752717SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Peptide chain release factor 1 (prfA) CSB-YP752718SSL
CSB-EP752718SSL
CSB-BP752718SSL
CSB-MP752718SSL
CSB-EP752718SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L36 (rpmJ) CSB-YP752719SSL
CSB-EP752719SSL
CSB-BP752719SSL
CSB-MP752719SSL
CSB-EP752719SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L5 (rplE) CSB-YP752720SSL
CSB-EP752720SSL
CSB-BP752720SSL
CSB-MP752720SSL
CSB-EP752720SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S19 (rpsS) CSB-YP752721SSL
CSB-EP752721SSL
CSB-BP752721SSL
CSB-MP752721SSL
CSB-EP752721SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP752722SSL
CSB-EP752722SSL
CSB-BP752722SSL
CSB-MP752722SSL
CSB-EP752722SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. S-adenosylmethionine synthase (metK) CSB-YP752723SSL
CSB-EP752723SSL
CSB-BP752723SSL
CSB-MP752723SSL
CSB-EP752723SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP752724SSL
CSB-EP752724SSL
CSB-BP752724SSL
CSB-MP752724SSL
CSB-EP752724SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP752725SSL
CSB-EP752725SSL
CSB-BP752725SSL
CSB-MP752725SSL
CSB-EP752725SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP752726SSL1
CSB-EP752726SSL1
CSB-BP752726SSL1
CSB-MP752726SSL1
CSB-EP752726SSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate--tRNA ligase (gltX) CSB-YP752727SSL
CSB-EP752727SSL
CSB-BP752727SSL
CSB-MP752727SSL
CSB-EP752727SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Transaldolase (tal) CSB-YP752728SSL
CSB-EP752728SSL
CSB-BP752728SSL
CSB-MP752728SSL
CSB-EP752728SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP752729SSL
CSB-EP752729SSL
CSB-BP752729SSL
CSB-MP752729SSL
CSB-EP752729SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor 4 (lepA), partial CSB-YP752730SSL
CSB-EP752730SSL
CSB-BP752730SSL
CSB-MP752730SSL
CSB-EP752730SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP752731SSL
CSB-EP752731SSL
CSB-BP752731SSL
CSB-MP752731SSL
CSB-EP752731SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase (hisG) CSB-YP752732SSL
CSB-EP752732SSL
CSB-BP752732SSL
CSB-MP752732SSL
CSB-EP752732SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP752733SSL
CSB-EP752733SSL
CSB-BP752733SSL
CSB-MP752733SSL
CSB-EP752733SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP752734SSL
CSB-EP752734SSL
CSB-BP752734SSL
CSB-MP752734SSL
CSB-EP752734SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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