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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mouse Protein SPATIAL (Spatial) CSB-YP752655MO
CSB-EP752655MO
CSB-BP752655MO
CSB-MP752655MO
CSB-EP752655MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SH3 domain-containing protein 21 (Sh3d21) CSB-YP752656MO
CSB-EP752656MO
CSB-BP752656MO
CSB-MP752656MO
CSB-EP752656MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pleckstrin homology domain-containing family M member 1 (Plekhm1), partial CSB-YP752657MO
CSB-EP752657MO
CSB-BP752657MO
CSB-MP752657MO
CSB-EP752657MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CMRF35-like molecule (Clm), partial CSB-YP752658MO
CSB-EP752658MO
CSB-BP752658MO
CSB-MP752658MO
CSB-EP752658MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial (Pdpr) CSB-YP752659MO
CSB-EP752659MO
CSB-BP752659MO
CSB-MP752659MO
CSB-EP752659MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zinc finger MYND domain-containing protein 19 (Zmynd19) CSB-YP752660RA
CSB-EP752660RA
CSB-BP752660RA
CSB-MP752660RA
CSB-EP752660RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zona pellucida sperm-binding protein 3 receptor (Zp3r) CSB-YP752661RA
CSB-EP752661RA
CSB-BP752661RA
CSB-MP752661RA
CSB-EP752661RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C20orf194 homolog, partial CSB-YP752662MO
CSB-EP752662MO
CSB-BP752662MO
CSB-MP752662MO
CSB-EP752662MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Paraplegin (Spg7), partial CSB-YP752663RA1
CSB-EP752663RA1
CSB-BP752663RA1
CSB-MP752663RA1
CSB-EP752663RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Elongation factor Tu (tufA) CSB-YP752664HSY
CSB-EP752664HSY
CSB-BP752664HSY
CSB-MP752664HSY
CSB-EP752664HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD32 (fadD32), partial CSB-YP752665MVH
CSB-EP752665MVH
CSB-BP752665MVH
CSB-MP752665MVH
CSB-EP752665MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP752666SSL
CSB-EP752666SSL
CSB-BP752666SSL
CSB-MP752666SSL
CSB-EP752666SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Anthranilate phosphoribosyltransferase (trpD) CSB-YP752667SSL
CSB-EP752667SSL
CSB-BP752667SSL
CSB-MP752667SSL
CSB-EP752667SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 10 kDa chaperonin (groS) CSB-YP752668SSL
CSB-EP752668SSL
CSB-BP752668SSL
CSB-MP752668SSL
CSB-EP752668SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP752669RDR
CSB-EP752669RDR
CSB-BP752669RDR
CSB-MP752669RDR
CSB-EP752669RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP752670EYQ
CSB-EP752670EYQ
CSB-BP752670EYQ
CSB-MP752670EYQ
CSB-EP752670EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP synthase epsilon chain (atpC) CSB-YP752671EYQ
CSB-EP752671EYQ
CSB-BP752671EYQ
CSB-MP752671EYQ
CSB-EP752671EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Bifunctional protein FolD (folD) CSB-YP752672EYQ
CSB-EP752672EYQ
CSB-BP752672EYQ
CSB-MP752672EYQ
CSB-EP752672EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP752673EYQ
CSB-EP752673EYQ
CSB-BP752673EYQ
CSB-MP752673EYQ
CSB-EP752673EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP752674EYQ
CSB-EP752674EYQ
CSB-BP752674EYQ
CSB-MP752674EYQ
CSB-EP752674EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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