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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Heliocidaris erythrogramma Homeobox protein orthopedia (Otp) CSB-YP754357HCW
CSB-EP754357HCW
CSB-BP754357HCW
CSB-MP754357HCW
CSB-EP754357HCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (SMARCD3) CSB-YP754358HU
CSB-EP754358HU
CSB-BP754358HU
CSB-MP754358HU
CSB-EP754358HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Deoxyuridine 5'-triphosphate nucleotidohydrolase (UL72) CSB-YP754359HWW
CSB-EP754359HWW
CSB-BP754359HWW
CSB-MP754359HWW
CSB-EP754359HWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ammi majus Caffeic acid 3-O-methyltransferase (COMT) CSB-YP754360AFAU
CSB-EP754360AFAU
CSB-BP754360AFAU
CSB-MP754360AFAU
CSB-EP754360AFAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cat 1,4-alpha-glucan-branching enzyme (GBE1), partial CSB-YP754361CA
CSB-EP754361CA
CSB-BP754361CA
CSB-MP754361CA
CSB-EP754361CA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Spermatogenesis-associated protein 20 (Spata20) CSB-YP754362RA
CSB-EP754362RA
CSB-BP754362RA
CSB-MP754362RA
CSB-EP754362RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lipid phosphate phosphatase-related protein type 3 (LPPR3), partial CSB-YP754363HU1
CSB-EP754363HU1
CSB-BP754363HU1
CSB-MP754363HU1
CSB-EP754363HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica Venom serine proteinase-like protein 1 CSB-YP754364BGW
CSB-EP754364BGW
CSB-BP754364BGW
CSB-MP754364BGW
CSB-EP754364BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica C-type lectin 2 CSB-YP754365BGW
CSB-EP754365BGW
CSB-BP754365BGW
CSB-MP754365BGW
CSB-EP754365BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoca largha Sex-determining region Y protein (SRY) CSB-YP754366PCAP
CSB-EP754366PCAP
CSB-BP754366PCAP
CSB-MP754366PCAP
CSB-EP754366PCAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pusa caspica Sex-determining region Y protein (SRY) CSB-YP754367PCAE
CSB-EP754367PCAE
CSB-BP754367PCAE
CSB-MP754367PCAE
CSB-EP754367PCAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monachus schauinslandi Sex-determining region Y protein (SRY) CSB-YP754368MEAY
CSB-EP754368MEAY
CSB-BP754368MEAY
CSB-MP754368MEAY
CSB-EP754368MEAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cat Sex-determining region Y protein (SRY) CSB-YP754369CA
CSB-EP754369CA
CSB-BP754369CA
CSB-MP754369CA
CSB-EP754369CA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Progestin and adipoQ receptor family member 6 (PAQR6), partial CSB-YP754370HU1
CSB-EP754370HU1
CSB-BP754370HU1
CSB-MP754370HU1
CSB-EP754370HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cancer susceptibility candidate protein 1 (CASC1) CSB-YP754371HU
CSB-EP754371HU
CSB-BP754371HU
CSB-MP754371HU
CSB-EP754371HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vitamin K epoxide reductase complex subunit 1-like protein 1 (Vkorc1l1), partial CSB-YP754372MO1
CSB-EP754372MO1
CSB-BP754372MO1
CSB-MP754372MO1
CSB-EP754372MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM91A1 (fam91a1), partial CSB-YP754373DIL
CSB-EP754373DIL
CSB-BP754373DIL
CSB-MP754373DIL
CSB-EP754373DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus gattii Autophagy-related protein 9 (ATG9), partial CSB-YP754374CHAY1
CSB-EP754374CHAY1
CSB-BP754374CHAY1
CSB-MP754374CHAY1
CSB-EP754374CHAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio STAM-binding protein-like A (stambpa) CSB-YP754375DIL
CSB-EP754375DIL
CSB-BP754375DIL
CSB-MP754375DIL
CSB-EP754375DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela putorius furo D (4) dopamine receptor (DRD4), partial CSB-YP754376MOH1
CSB-EP754376MOH1
CSB-BP754376MOH1
CSB-MP754376MOH1
CSB-EP754376MOH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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