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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopirellula baltica Aspartate carbamoyltransferase (pyrB) CSB-YP756163RDR
CSB-EP756163RDR
CSB-BP756163RDR
CSB-MP756163RDR
CSB-EP756163RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Phosphomethylpyrimidine synthase (thiC) CSB-YP756164RDR
CSB-EP756164RDR
CSB-BP756164RDR
CSB-MP756164RDR
CSB-EP756164RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP756165RDR
CSB-EP756165RDR
CSB-BP756165RDR
CSB-MP756165RDR
CSB-EP756165RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Chorismate synthase (aroC) CSB-YP756166RDR
CSB-EP756166RDR
CSB-BP756166RDR
CSB-MP756166RDR
CSB-EP756166RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Dephospho-CoA kinase (coaE) CSB-YP756167RDR
CSB-EP756167RDR
CSB-BP756167RDR
CSB-MP756167RDR
CSB-EP756167RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0365 protein RB6389 (RB6389), partial CSB-YP756168RDR1
CSB-EP756168RDR1
CSB-BP756168RDR1
CSB-MP756168RDR1
CSB-EP756168RDR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Trigger factor (tig) CSB-YP756169RDR
CSB-EP756169RDR
CSB-BP756169RDR
CSB-MP756169RDR
CSB-EP756169RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Methionine--tRNA ligase (metG), partial CSB-YP756170RDR
CSB-EP756170RDR
CSB-BP756170RDR
CSB-MP756170RDR
CSB-EP756170RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Cysteine--tRNA ligase (cysS), partial CSB-YP756171RDR
CSB-EP756171RDR
CSB-BP756171RDR
CSB-MP756171RDR
CSB-EP756171RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Carboxylate-amine ligase RB4485 (RB4485) CSB-YP756172RDR
CSB-EP756172RDR
CSB-BP756172RDR
CSB-MP756172RDR
CSB-EP756172RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glycerol kinase (glpK), partial CSB-YP756173RDR
CSB-EP756173RDR
CSB-BP756173RDR
CSB-MP756173RDR
CSB-EP756173RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP756174RDR
CSB-EP756174RDR
CSB-BP756174RDR
CSB-MP756174RDR
CSB-EP756174RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Transcription elongation factor GreA (greA) CSB-YP756175RDR
CSB-EP756175RDR
CSB-BP756175RDR
CSB-MP756175RDR
CSB-EP756175RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica GTPase obg (obg) CSB-YP756176RDR
CSB-EP756176RDR
CSB-BP756176RDR
CSB-MP756176RDR
CSB-EP756176RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Protein translocase subunit SecA 2 (secA2), partial CSB-YP756177RDR
CSB-EP756177RDR
CSB-BP756177RDR
CSB-MP756177RDR
CSB-EP756177RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Lipoprotein-releasing system ATP-binding protein LolD 1 (lolD1) CSB-YP756178RDR
CSB-EP756178RDR
CSB-BP756178RDR
CSB-MP756178RDR
CSB-EP756178RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0753 protein RB1129 (RB1129), partial CSB-YP756179RDR
CSB-EP756179RDR
CSB-BP756179RDR
CSB-MP756179RDR
CSB-EP756179RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Probable molybdenum cofactor guanylyltransferase (mobA) CSB-YP756180RDR
CSB-EP756180RDR
CSB-BP756180RDR
CSB-MP756180RDR
CSB-EP756180RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 7-cyano-7-deazaguanine synthase (queC) CSB-YP756181EYQ
CSB-EP756181EYQ
CSB-BP756181EYQ
CSB-MP756181EYQ
CSB-EP756181EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L35 (rpmI) CSB-YP756182EYQ
CSB-EP756182EYQ
CSB-BP756182EYQ
CSB-MP756182EYQ
CSB-EP756182EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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