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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus ducreyi 30S ribosomal protein S2 (rpsB) CSB-YP763521HSY
CSB-EP763521HSY
CSB-BP763521HSY
CSB-MP763521HSY
CSB-EP763521HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Chromosome partition protein MukB (mukB), partial CSB-YP763522HSY
CSB-EP763522HSY
CSB-BP763522HSY
CSB-MP763522HSY
CSB-EP763522HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP763523HSY
CSB-EP763523HSY
CSB-BP763523HSY
CSB-MP763523HSY
CSB-EP763523HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Asparagine--tRNA ligase (asnS) CSB-YP763524HSY
CSB-EP763524HSY
CSB-BP763524HSY
CSB-MP763524HSY
CSB-EP763524HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi tRNA (Met) cytidine acetyltransferase TmcA (tmcA), partial CSB-YP763525HSY
CSB-EP763525HSY
CSB-BP763525HSY
CSB-MP763525HSY
CSB-EP763525HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi UPF0307 protein HD_1362 (HD_1362) CSB-YP763526HSY
CSB-EP763526HSY
CSB-BP763526HSY
CSB-MP763526HSY
CSB-EP763526HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP763527HSY
CSB-EP763527HSY
CSB-BP763527HSY
CSB-MP763527HSY
CSB-EP763527HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi tRNA (cmo5U34)-methyltransferase (cmoA) CSB-YP763528HSY
CSB-EP763528HSY
CSB-BP763528HSY
CSB-MP763528HSY
CSB-EP763528HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Outer-membrane lipoprotein carrier protein (lolA) CSB-YP763529HSY
CSB-EP763529HSY
CSB-BP763529HSY
CSB-MP763529HSY
CSB-EP763529HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi GTPase Der (der), partial CSB-YP763530HSY
CSB-EP763530HSY
CSB-BP763530HSY
CSB-MP763530HSY
CSB-EP763530HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP763531HSY
CSB-EP763531HSY
CSB-BP763531HSY
CSB-MP763531HSY
CSB-EP763531HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi DNA gyrase inhibitor YacG (yacG) CSB-YP763532HSY
CSB-EP763532HSY
CSB-BP763532HSY
CSB-MP763532HSY
CSB-EP763532HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Cysteine desulfurase (iscS) CSB-YP763533HSY
CSB-EP763533HSY
CSB-BP763533HSY
CSB-MP763533HSY
CSB-EP763533HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Nucleoside diphosphate kinase (ndk) CSB-YP763534HSY
CSB-EP763534HSY
CSB-BP763534HSY
CSB-MP763534HSY
CSB-EP763534HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP763535HSY
CSB-EP763535HSY
CSB-BP763535HSY
CSB-MP763535HSY
CSB-EP763535HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi UPF0263 protein HD_0986 (HD_0986) CSB-YP763536HSY
CSB-EP763536HSY
CSB-BP763536HSY
CSB-MP763536HSY
CSB-EP763536HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Phosphoglycerate kinase (pgk) CSB-YP763537HSY
CSB-EP763537HSY
CSB-BP763537HSY
CSB-MP763537HSY
CSB-EP763537HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP763538HSY
CSB-EP763538HSY
CSB-BP763538HSY
CSB-MP763538HSY
CSB-EP763538HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Penicillin-binding protein activator LpoA (lpoA), partial CSB-YP763539HSY
CSB-EP763539HSY
CSB-BP763539HSY
CSB-MP763539HSY
CSB-EP763539HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 5'-nucleotidase surE (surE) CSB-YP763540HSY
CSB-EP763540HSY
CSB-BP763540HSY
CSB-MP763540HSY
CSB-EP763540HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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