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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP763561BHM
CSB-EP763561BHM
CSB-BP763561BHM
CSB-MP763561BHM
CSB-EP763561BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP763562BHM
CSB-EP763562BHM
CSB-BP763562BHM
CSB-MP763562BHM
CSB-EP763562BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L3 (rplC) CSB-YP763563BHM
CSB-EP763563BHM
CSB-BP763563BHM
CSB-MP763563BHM
CSB-EP763563BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine--tRNA ligase (alaS), partial CSB-YP763564BHM
CSB-EP763564BHM
CSB-BP763564BHM
CSB-MP763564BHM
CSB-EP763564BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP763565BHM
CSB-EP763565BHM
CSB-BP763565BHM
CSB-MP763565BHM
CSB-EP763565BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydrogenase (leuB) CSB-YP763566BHM
CSB-EP763566BHM
CSB-BP763566BHM
CSB-MP763566BHM
CSB-EP763566BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP763567BHM
CSB-EP763567BHM
CSB-BP763567BHM
CSB-MP763567BHM
CSB-EP763567BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP763568BHM
CSB-EP763568BHM
CSB-BP763568BHM
CSB-MP763568BHM
CSB-EP763568BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligoribonuclease (orn) CSB-YP763569BHM
CSB-EP763569BHM
CSB-BP763569BHM
CSB-MP763569BHM
CSB-EP763569BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S9 (rpsI) CSB-YP763570BHM
CSB-EP763570BHM
CSB-BP763570BHM
CSB-MP763570BHM
CSB-EP763570BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein insertion efficiency factor (Bfl013) CSB-YP763571BHM
CSB-EP763571BHM
CSB-BP763571BHM
CSB-MP763571BHM
CSB-EP763571BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP763572BHM1
CSB-EP763572BHM1
CSB-BP763572BHM1
CSB-MP763572BHM1
CSB-EP763572BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP763573BHM
CSB-EP763573BHM
CSB-BP763573BHM
CSB-MP763573BHM
CSB-EP763573BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable septum site-determining protein MinC (minC) CSB-YP763574BHM
CSB-EP763574BHM
CSB-BP763574BHM
CSB-MP763574BHM
CSB-EP763574BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP763575BHM1
CSB-EP763575BHM1
CSB-BP763575BHM1
CSB-MP763575BHM1
CSB-EP763575BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP763576BHM
CSB-EP763576BHM
CSB-BP763576BHM
CSB-MP763576BHM
CSB-EP763576BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP763577BHM
CSB-EP763577BHM
CSB-BP763577BHM
CSB-MP763577BHM
CSB-EP763577BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoate-protein ligase A (lplA) CSB-YP763578BHM
CSB-EP763578BHM
CSB-BP763578BHM
CSB-MP763578BHM
CSB-EP763578BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP763579BHM
CSB-EP763579BHM
CSB-BP763579BHM
CSB-MP763579BHM
CSB-EP763579BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP763580BHM
CSB-EP763580BHM
CSB-BP763580BHM
CSB-MP763580BHM
CSB-EP763580BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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