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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Guanylate cyclase 2G (Gucy2g), partial CSB-YP764902MO
CSB-EP764902MO
CSB-BP764902MO
CSB-MP764902MO
CSB-EP764902MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum SrfA-induced gene F protein (sigF) CSB-YP764903DKK
CSB-EP764903DKK
CSB-BP764903DKK
CSB-MP764903DKK
CSB-EP764903DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Siniperca chuatsi Interferon-induced GTP-binding protein Mx (mx), partial CSB-YP764904STU
CSB-EP764904STU
CSB-BP764904STU
CSB-MP764904STU
CSB-EP764904STU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Flap endonuclease 1 (fen1) CSB-YP764905DIL
CSB-EP764905DIL
CSB-BP764905DIL
CSB-MP764905DIL
CSB-EP764905DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-defensin 50 (Defb50) CSB-YP764906MO
CSB-EP764906MO
CSB-BP764906MO
CSB-MP764906MO
CSB-EP764906MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yaba monkey tumor virus Poly (A) polymerase catalytic subunit (PAPL) CSB-YP764907YAA
CSB-EP764907YAA
CSB-BP764907YAA
CSB-MP764907YAA
CSB-EP764907YAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat-containing protein 66 (Lrrc66), partial CSB-YP764908RA
CSB-EP764908RA
CSB-BP764908RA
CSB-MP764908RA
CSB-EP764908RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Gap junction alpha-1 protein (GJA1), partial CSB-YP764909DSU1
CSB-EP764909DSU1
CSB-BP764909DSU1
CSB-MP764909DSU1
CSB-EP764909DSU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zinc finger CCCH domain-containing protein 15 (Zc3h15) CSB-YP764910RA
CSB-EP764910RA
CSB-BP764910RA
CSB-MP764910RA
CSB-EP764910RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial CSB-YP764911CH
CSB-EP764911CH
CSB-BP764911CH
CSB-MP764911CH
CSB-EP764911CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Forkhead box protein P3 (FOXP3) CSB-YP764912MOV
CSB-EP764912MOV
CSB-BP764912MOV
CSB-MP764912MOV
CSB-EP764912MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ankyrin repeat domain-containing protein 12 (ANKRD12), partial CSB-YP764913HU
CSB-EP764913HU
CSB-BP764913HU
CSB-MP764913HU
CSB-EP764913HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious salmon anemia virus Polymerase basic protein 2 (PB2), partial CSB-YP764914ICAH
CSB-EP764914ICAH
CSB-BP764914ICAH
CSB-MP764914ICAH
CSB-EP764914ICAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Envelope glycoprotein I (US7), partial CSB-YP764915PAAF1
CSB-EP764915PAAF1
CSB-BP764915PAAF1
CSB-MP764915PAAF1
CSB-EP764915PAAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Uncharacterized protein US2 (US2) CSB-YP764916PAAF
CSB-EP764916PAAF
CSB-BP764916PAAF
CSB-MP764916PAAF
CSB-EP764916PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Uncharacterized protein UL3 (UL3) CSB-YP764917PAAF
CSB-EP764917PAAF
CSB-BP764917PAAF
CSB-MP764917PAAF
CSB-EP764917PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Capsid-binding protein UL16 (UL16) CSB-YP764918PAAF
CSB-EP764918PAAF
CSB-BP764918PAAF
CSB-MP764918PAAF
CSB-EP764918PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Ribonucleoside-diphosphate reductase small chain (UL40) CSB-YP764919PAAF
CSB-EP764919PAAF
CSB-BP764919PAAF
CSB-MP764919PAAF
CSB-EP764919PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 Capsid assembly protein UL37 (UL37), partial CSB-YP764920PAAF
CSB-EP764920PAAF
CSB-BP764920PAAF
CSB-MP764920PAAF
CSB-EP764920PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psittacid herpesvirus 1 DNA polymerase catalytic subunit (UL30), partial CSB-YP764921PAAF
CSB-EP764921PAAF
CSB-BP764921PAAF
CSB-MP764921PAAF
CSB-EP764921PAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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