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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces anulatus Diaminobutyrate--2-oxoglutarate transaminase (ectB) CSB-YP764842SNK
CSB-EP764842SNK
CSB-BP764842SNK
CSB-MP764842SNK
CSB-EP764842SNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerochaete chrysosporium Pyranose 2-oxidase (p2ox), partial CSB-YP764843EUK
CSB-EP764843EUK
CSB-BP764843EUK
CSB-MP764843EUK
CSB-EP764843EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Transcription initiation factor TFIID subunit 7 (TAF7) CSB-YP764844DXU
CSB-EP764844DXU
CSB-BP764844DXU
CSB-MP764844DXU
CSB-EP764844DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable metal-nicotianamine transporter YSL4 (YSL4), partial CSB-YP764845DOA
CSB-EP764845DOA
CSB-BP764845DOA
CSB-MP764845DOA
CSB-EP764845DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Toll-like receptor 11 (Tlr11), partial CSB-YP764846MO
CSB-BP764846MO
CSB-MP764846MO
CSB-EP764846MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Relaxin receptor 1 (Rxfp1), partial CSB-YP764847MO1
CSB-EP764847MO1
CSB-BP764847MO1
CSB-MP764847MO1
CSB-EP764847MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF120 (ORF120) CSB-YP764848OBAD
CSB-EP764848OBAD
CSB-BP764848OBAD
CSB-MP764848OBAD
CSB-EP764848OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF2 (ORF2) CSB-YP764849OBAD
CSB-EP764849OBAD
CSB-BP764849OBAD
CSB-MP764849OBAD
CSB-EP764849OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF102 (ORF102), partial CSB-YP764850OBAD
CSB-EP764850OBAD
CSB-BP764850OBAD
CSB-MP764850OBAD
CSB-EP764850OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF93 (ORF93) CSB-YP764851OBAD
CSB-EP764851OBAD
CSB-BP764851OBAD
CSB-MP764851OBAD
CSB-EP764851OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Putative apoptosis inhibitor ORF87 (ORF87) CSB-YP764852OBAD
CSB-EP764852OBAD
CSB-BP764852OBAD
CSB-MP764852OBAD
CSB-EP764852OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF83 (ORF83) CSB-YP764853OBAD
CSB-EP764853OBAD
CSB-BP764853OBAD
CSB-MP764853OBAD
CSB-EP764853OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF78 (ORF78), partial CSB-YP764854OBAD
CSB-EP764854OBAD
CSB-BP764854OBAD
CSB-MP764854OBAD
CSB-EP764854OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF75 (ORF75) CSB-YP764855OBAD
CSB-EP764855OBAD
CSB-BP764855OBAD
CSB-MP764855OBAD
CSB-EP764855OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF67 (ORF67), partial CSB-YP764856OBAD
CSB-EP764856OBAD
CSB-BP764856OBAD
CSB-MP764856OBAD
CSB-EP764856OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Putative transmembrane protein ORF57 (ORF57), partial CSB-YP764857OBAD1
CSB-EP764857OBAD1
CSB-BP764857OBAD1
CSB-MP764857OBAD1
CSB-EP764857OBAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Putative RING finger protein ORF38 (ORF38) CSB-YP764858OBAD
CSB-EP764858OBAD
CSB-BP764858OBAD
CSB-MP764858OBAD
CSB-EP764858OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF33 (ORF33) CSB-YP764859OBAD
CSB-EP764859OBAD
CSB-BP764859OBAD
CSB-MP764859OBAD
CSB-EP764859OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF28 (ORF28), partial CSB-YP764860OBAD
CSB-EP764860OBAD
CSB-BP764860OBAD
CSB-MP764860OBAD
CSB-EP764860OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF21 (ORF21), partial CSB-YP764861OBAD
CSB-EP764861OBAD
CSB-BP764861OBAD
CSB-MP764861OBAD
CSB-EP764861OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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