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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Anguilla japonica Galactose-binding lectin l-1 (l-1) CSB-YP772092BZH
CSB-EP772092BZH
CSB-BP772092BZH
CSB-MP772092BZH
CSB-EP772092BZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius halys Snake venom serine protease PTLE1 CSB-YP772093GGQ
CSB-EP772093GGQ
CSB-BP772093GGQ
CSB-MP772093GGQ
CSB-EP772093GGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (ALG6), partial CSB-YP772094CH
CSB-EP772094CH
CSB-BP772094CH
CSB-MP772094CH
CSB-EP772094CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio O-phosphoseryl-tRNA (Sec) selenium transferase (sepsecs) CSB-YP772095DIL
CSB-EP772095DIL
CSB-BP772095DIL
CSB-MP772095DIL
CSB-EP772095DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lissencephaly-1 homolog B (pafah1b1b) CSB-YP772096DIL
CSB-EP772096DIL
CSB-BP772096DIL
CSB-MP772096DIL
CSB-EP772096DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase RING2 (rnf2) CSB-YP772097DIL
CSB-EP772097DIL
CSB-BP772097DIL
CSB-MP772097DIL
CSB-EP772097DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Eukaryotic translation initiation factor 3 subunit J-B (eif3jb) CSB-YP772098DIL
CSB-EP772098DIL
CSB-BP772098DIL
CSB-MP772098DIL
CSB-EP772098DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM40A (fam40a), partial CSB-YP772099DIL
CSB-EP772099DIL
CSB-BP772099DIL
CSB-MP772099DIL
CSB-EP772099DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein YIPF3 (yipf3), partial CSB-YP772100DIL1
CSB-EP772100DIL1
CSB-BP772100DIL1
CSB-MP772100DIL1
CSB-EP772100DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein EMX1 (emx1) CSB-YP772101DIL
CSB-EP772101DIL
CSB-BP772101DIL
CSB-MP772101DIL
CSB-EP772101DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Phospholipase A2 PLA-N/PLA-N (O) CSB-YP772102EZB
CSB-EP772102EZB
CSB-BP772102EZB
CSB-MP772102EZB
CSB-EP772102EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Natriuretic peptides A (nppa) CSB-YP772103TKY
CSB-EP772103TKY
CSB-BP772103TKY
CSB-MP772103TKY
CSB-EP772103TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agkistrodon contortrix contortrix Zinc metalloproteinase/disintegrin CSB-YP772104AET
CSB-EP772104AET
CSB-BP772104AET
CSB-MP772104AET
CSB-EP772104AET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine circovirus 1 Replication-associated protein (Rep) CSB-YP772105PIAM
CSB-EP772105PIAM
CSB-BP772105PIAM
CSB-MP772105PIAM
CSB-EP772105PIAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized protein VACWR003 (VACWR003) CSB-YP772106VAI
CSB-EP772106VAI
CSB-BP772106VAI
CSB-MP772106VAI
CSB-EP772106VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP772107HNJ
CSB-EP772107HNJ
CSB-BP772107HNJ
CSB-MP772107HNJ
CSB-EP772107HNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E7 (E7) CSB-YP772108HNQ
CSB-EP772108HNQ
CSB-BP772108HNQ
CSB-MP772108HNQ
CSB-EP772108HNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP772109HNT
CSB-EP772109HNT
CSB-BP772109HNT
CSB-MP772109HNT
CSB-EP772109HNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2) CSB-YP772110HOB
CSB-EP772110HOB
CSB-BP772110HOB
CSB-MP772110HOB
CSB-EP772110HOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP772111HOH
CSB-EP772111HOH
CSB-BP772111HOH
CSB-MP772111HOH
CSB-EP772111HOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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