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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Cullin-9 (Cul9), partial CSB-YP772132MO
CSB-EP772132MO
CSB-BP772132MO
CSB-MP772132MO
CSB-EP772132MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase Praja-2 (Pja2) CSB-YP772133MO
CSB-EP772133MO
CSB-BP772133MO
CSB-MP772133MO
CSB-EP772133MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein CLEC16A (Clec16a), partial CSB-YP772134MO
CSB-EP772134MO
CSB-BP772134MO
CSB-MP772134MO
CSB-EP772134MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Regulating synaptic membrane exocytosis protein 3 (Rims3) CSB-YP772135MO
CSB-EP772135MO
CSB-BP772135MO
CSB-MP772135MO
CSB-EP772135MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein scribble homolog (Scrib), partial CSB-YP772136MO
CSB-EP772136MO
CSB-BP772136MO
CSB-MP772136MO
CSB-EP772136MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase WNK4 (Wnk4), partial CSB-YP772137MO
CSB-EP772137MO
CSB-BP772137MO
CSB-MP772137MO
CSB-EP772137MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ALK and LTK ligand 2 (Alkal2) CSB-YP772138MO
CSB-EP772138MO
CSB-BP772138MO
CSB-MP772138MO
CSB-EP772138MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SEC14 domain and spectrin repeat-containing protein 1 (Sestd1) CSB-YP772139MO
CSB-EP772139MO
CSB-BP772139MO
CSB-MP772139MO
CSB-EP772139MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Diacylglycerol kinase zeta (Dgkz), partial CSB-YP772140MO
CSB-EP772140MO
CSB-BP772140MO
CSB-MP772140MO
CSB-EP772140MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Membrane-associated progesterone receptor component 2 (Pgrmc2), partial CSB-YP772141MO1
CSB-EP772141MO1
CSB-BP772141MO1
CSB-MP772141MO1
CSB-EP772141MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Abhydrolase domain-containing protein 13 (Abhd13), partial CSB-YP772142MO1
CSB-EP772142MO1
CSB-BP772142MO1
CSB-MP772142MO1
CSB-EP772142MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription cofactor vestigial-like protein 4 (Vgll4) CSB-YP772143MO
CSB-EP772143MO
CSB-BP772143MO
CSB-MP772143MO
CSB-EP772143MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse PR domain zinc finger protein 4 (Prdm4), partial CSB-YP772144MO
CSB-EP772144MO
CSB-BP772144MO
CSB-MP772144MO
CSB-EP772144MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse AP-4 complex subunit epsilon-1 (Ap4e1), partial CSB-YP772145MO
CSB-EP772145MO
CSB-BP772145MO
CSB-MP772145MO
CSB-EP772145MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sex comb on midleg-like protein 4 (Scml4) CSB-YP772146MO
CSB-EP772146MO
CSB-BP772146MO
CSB-MP772146MO
CSB-EP772146MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho guanine nucleotide exchange factor 38 (Arhgef38) CSB-YP772147MO
CSB-EP772147MO
CSB-BP772147MO
CSB-MP772147MO
CSB-EP772147MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Otopetrin-1 (Otop1), partial CSB-YP772148MO
CSB-EP772148MO
CSB-BP772148MO
CSB-MP772148MO
CSB-EP772148MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Aspartate beta-hydroxylase domain-containing protein 2 (Asphd2), partial CSB-YP772149MO
CSB-EP772149MO
CSB-BP772149MO
CSB-MP772149MO
CSB-EP772149MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Whirlin (Whrn), partial CSB-YP772150MO
CSB-EP772150MO
CSB-BP772150MO
CSB-MP772150MO
CSB-EP772150MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glutathione S-transferase Mu 7 (Gstm7) CSB-YP772151MO
CSB-EP772151MO
CSB-BP772151MO
CSB-MP772151MO
CSB-EP772151MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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