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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana ABC transporter G family member 25 (ABCG25), partial CSB-YP774612DOA1
CSB-EP774612DOA1
CSB-BP774612DOA1
CSB-MP774612DOA1
CSB-EP774612DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 4, chloroplastic (petD), partial CSB-YP774613EHU1
CSB-EP774613EHU1
CSB-BP774613EHU1
CSB-MP774613EHU1
CSB-EP774613EHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable calcium-binding protein CML32 (CML32) CSB-YP774614OFG
CSB-EP774614OFG
CSB-BP774614OFG
CSB-MP774614OFG
CSB-EP774614OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CASP-like protein BLE3 (BLE3), partial CSB-YP774615OFG1
CSB-EP774615OFG1
CSB-BP774615OFG1
CSB-MP774615OFG1
CSB-EP774615OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Serine/threonine-protein kinase stt7, chloroplastic (STT7), partial CSB-YP774616DSJ
CSB-EP774616DSJ
CSB-BP774616DSJ
CSB-MP774616DSJ
CSB-EP774616DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable calcium-binding protein CML7 (CML7) CSB-YP774617OFG
CSB-EP774617OFG
CSB-BP774617OFG
CSB-MP774617OFG
CSB-EP774617OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aberrant root formation protein 4 (ALF4), partial CSB-YP774618DOA
CSB-EP774618DOA
CSB-BP774618DOA
CSB-MP774618DOA
CSB-EP774618DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Type II inositol 1,4,5-trisphosphate 5-phosphatase FRA3 (FRA3), partial CSB-YP774619DOA
CSB-EP774619DOA
CSB-BP774619DOA
CSB-MP774619DOA
CSB-EP774619DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 32 (At2g47850) CSB-YP774620DOA
CSB-EP774620DOA
CSB-BP774620DOA
CSB-MP774620DOA
CSB-EP774620DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable VAMP-like protein At1g33475 (At1g33475), partial CSB-YP774621DOA1
CSB-EP774621DOA1
CSB-BP774621DOA1
CSB-MP774621DOA1
CSB-EP774621DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pectinesterase PPME1 (PPME1) CSB-YP774622DOA
CSB-EP774622DOA
CSB-BP774622DOA
CSB-MP774622DOA
CSB-EP774622DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine proteinase inhibitor 4 (CYS4) CSB-YP774623DOA
CSB-EP774623DOA
CSB-BP774623DOA
CSB-MP774623DOA
CSB-EP774623DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024) CSB-YP774624DOA
CSB-EP774624DOA
CSB-BP774624DOA
CSB-MP774624DOA
CSB-EP774624DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box only protein 8 (FBX8) CSB-YP774625DOA
CSB-EP774625DOA
CSB-BP774625DOA
CSB-MP774625DOA
CSB-EP774625DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CAX-interacting protein 4 (CXIP4) CSB-YP774626DOA
CSB-EP774626DOA
CSB-BP774626DOA
CSB-MP774626DOA
CSB-EP774626DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative xyloglucan glycosyltransferase 10 (CSLC10), partial CSB-YP774627OFG1
CSB-EP774627OFG1
CSB-BP774627OFG1
CSB-MP774627OFG1
CSB-EP774627OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemisia vulgaris Major pollen allergen Art v 1 CSB-YP774628AOH
CSB-BP774628AOH
CSB-MP774628AOH
CSB-EP774628AOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-like B1 (EXLB1) CSB-YP774629OFG
CSB-EP774629OFG
CSB-BP774629OFG
CSB-MP774629OFG
CSB-EP774629OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 3-6 (Os03g0694000, LOC_Os03g48780) CSB-YP774630OFG
CSB-EP774630OFG
CSB-BP774630OFG
CSB-MP774630OFG
CSB-EP774630OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os03g0620400 (Os03g0620400, LOC_Os03g42280), partial CSB-YP774631OFG
CSB-EP774631OFG
CSB-BP774631OFG
CSB-MP774631OFG
CSB-EP774631OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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