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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa ATPase get3 (get3) CSB-YP801274NHA
CSB-EP801274NHA
CSB-BP801274NHA
CSB-MP801274NHA
CSB-EP801274NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Autophagy-related protein 2 (atg-2), partial CSB-YP801275NHA
CSB-EP801275NHA
CSB-BP801275NHA
CSB-MP801275NHA
CSB-EP801275NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Glycosylphosphatidylinositol anchor biosynthesis protein 11 (gpi-11), partial CSB-YP801276NHA1
CSB-EP801276NHA1
CSB-BP801276NHA1
CSB-MP801276NHA1
CSB-EP801276NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative DNA helicase ino-80 (ino-80), partial CSB-YP801277NHA
CSB-EP801277NHA
CSB-BP801277NHA
CSB-MP801277NHA
CSB-EP801277NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein transport protein sec-31 (sec-31), partial CSB-YP801278NHA
CSB-EP801278NHA
CSB-BP801278NHA
CSB-MP801278NHA
CSB-EP801278NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone H2A.Z (htz-1) CSB-YP801279NHA
CSB-EP801279NHA
CSB-BP801279NHA
CSB-MP801279NHA
CSB-EP801279NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans GPI-anchored wall transfer protein 1 (GWT1), partial CSB-YP801280CZD
CSB-EP801280CZD
CSB-BP801280CZD
CSB-MP801280CZD
CSB-EP801280CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannitol-1-phosphate 5-dehydrogenase CSB-YP801281PBAS
CSB-EP801281PBAS
CSB-BP801281PBAS
CSB-MP801281PBAS
CSB-EP801281PBAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis Cytochrome b5 (Cytb5), partial CSB-YP801282CZN1
CSB-EP801282CZN1
CSB-BP801282CZN1
CSB-MP801282CZN1
CSB-EP801282CZN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cytosolic Fe-S cluster assembly factor NBP35 (NBP35) CSB-YP801283CZI
CSB-EP801283CZI
CSB-BP801283CZI
CSB-MP801283CZI
CSB-EP801283CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Glutamine synthetase (GLN1) CSB-YP801284KBK
CSB-EP801284KBK
CSB-BP801284KBK
CSB-MP801284KBK
CSB-EP801284KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Histone H2B (HTB1) CSB-YP801285EXI
CSB-EP801285EXI
CSB-BP801285EXI
CSB-MP801285EXI
CSB-EP801285EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hansenula anomala N- (5'-phosphoribosyl)anthranilate isomerase (TRP1) CSB-YP801286HUH
CSB-EP801286HUH
CSB-BP801286HUH
CSB-MP801286HUH
CSB-EP801286HUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Palmitoyltransferase AKR1 (AKR1), partial CSB-YP801287KBK1
CSB-EP801287KBK1
CSB-BP801287KBK1
CSB-MP801287KBK1
CSB-EP801287KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Elongation factor 2 (EFT2), partial CSB-YP801288LMA
CSB-EP801288LMA
CSB-BP801288LMA
CSB-MP801288LMA
CSB-EP801288LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naumovozyma castellii Elongation factor 2 (EFT1), partial CSB-YP801289NAY
CSB-EP801289NAY
CSB-BP801289NAY
CSB-MP801289NAY
CSB-EP801289NAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces bayanus Sporulation-specific glucan 1,3-beta-glucosidase (SPR1) CSB-YP801290SVF
CSB-EP801290SVF
CSB-BP801290SVF
CSB-MP801290SVF
CSB-EP801290SVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces bayanus Palmitoyltransferase AKR1 (AKR1), partial CSB-YP801291SVF1
CSB-EP801291SVF1
CSB-BP801291SVF1
CSB-MP801291SVF1
CSB-EP801291SVF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae Superoxide dismutase [Mn], mitochondrial (sodB), partial CSB-YP801292DPA
CSB-EP801292DPA
CSB-BP801292DPA
CSB-MP801292DPA
CSB-EP801292DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribonuclease P protein component (rnpA) CSB-YP801293XAT
CSB-EP801293XAT
CSB-BP801293XAT
CSB-MP801293XAT
CSB-EP801293XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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