Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase DDB_G0271682 (DDB_G0271682), partial CSB-YP803075DKK
CSB-EP803075DKK
CSB-BP803075DKK
CSB-MP803075DKK
CSB-EP803075DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase PLK (PLK), partial CSB-YP803076DKK
CSB-EP803076DKK
CSB-BP803076DKK
CSB-MP803076DKK
CSB-EP803076DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum BolA-like protein DDB_G0274439 (DDB_G0274439) CSB-YP803077DKK
CSB-EP803077DKK
CSB-BP803077DKK
CSB-MP803077DKK
CSB-EP803077DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum COMM domain-containing protein 7 (commd7) CSB-YP803078DKK
CSB-EP803078DKK
CSB-BP803078DKK
CSB-MP803078DKK
CSB-EP803078DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0275161 (DDB_G0275161) CSB-YP803079DKK
CSB-EP803079DKK
CSB-BP803079DKK
CSB-MP803079DKK
CSB-EP803079DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0271888 (DDB_G0271888) CSB-YP803080DKK
CSB-EP803080DKK
CSB-BP803080DKK
CSB-MP803080DKK
CSB-EP803080DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized FAM18-like protein 1 (DDB_G0276319), partial CSB-YP803081DKK1
CSB-EP803081DKK1
CSB-BP803081DKK1
CSB-MP803081DKK1
CSB-EP803081DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275485 (DDB_G0275485), partial CSB-YP803082DKK
CSB-EP803082DKK
CSB-BP803082DKK
CSB-MP803082DKK
CSB-EP803082DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum N-acetyltransferase 9-like protein (nat9) CSB-YP803083DKK
CSB-EP803083DKK
CSB-BP803083DKK
CSB-MP803083DKK
CSB-EP803083DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable protein tyrosine phosphatase type IVA B (DDB_G0272662) CSB-YP803084DKK
CSB-EP803084DKK
CSB-BP803084DKK
CSB-MP803084DKK
CSB-EP803084DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0274353 (DDB_G0274353) CSB-YP803085DKK
CSB-EP803085DKK
CSB-BP803085DKK
CSB-MP803085DKK
CSB-EP803085DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein phosphatase inhibitor 2 (dpiA) CSB-YP803086DKK
CSB-EP803086DKK
CSB-BP803086DKK
CSB-MP803086DKK
CSB-EP803086DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Frizzled and smoothened-like protein C (fslC), partial CSB-YP803087DKK1
CSB-EP803087DKK1
CSB-BP803087DKK1
CSB-MP803087DKK1
CSB-EP803087DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271558 (DDB_G0271558) CSB-YP803088DKK
CSB-EP803088DKK
CSB-BP803088DKK
CSB-MP803088DKK
CSB-EP803088DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DNA-directed RNA polymerase III subunit rpc6 (polr3f) CSB-YP803089DKK
CSB-EP803089DKK
CSB-BP803089DKK
CSB-MP803089DKK
CSB-EP803089DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable ubiquitin-conjugating enzyme E2 N (ube2n) CSB-YP803090DKK
CSB-EP803090DKK
CSB-BP803090DKK
CSB-MP803090DKK
CSB-EP803090DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0420 protein (DDB_G0277179), partial CSB-YP803091DKK1
CSB-EP803091DKK1
CSB-BP803091DKK1
CSB-MP803091DKK1
CSB-EP803091DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable GH family 25 lysozyme 2 (DDB_G0274181) CSB-YP803092DKK
CSB-EP803092DKK
CSB-BP803092DKK
CSB-MP803092DKK
CSB-EP803092DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Small heat shock protein hspG9 (hspG9) CSB-YP803093DKK
CSB-EP803093DKK
CSB-BP803093DKK
CSB-MP803093DKK
CSB-EP803093DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable E3 ubiquitin-protein ligase bre1 (bre1), partial CSB-YP803094DKK
CSB-EP803094DKK
CSB-BP803094DKK
CSB-MP803094DKK
CSB-EP803094DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>