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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP803475FGB
CSB-EP803475FGB
CSB-BP803475FGB
CSB-MP803475FGB
CSB-EP803475FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribonuclease Z (rnz) CSB-YP803476FGB
CSB-EP803476FGB
CSB-BP803476FGB
CSB-MP803476FGB
CSB-EP803476FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP803477FGB
CSB-EP803477FGB
CSB-BP803477FGB
CSB-MP803477FGB
CSB-EP803477FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Na (+)/H (+) antiporter nhaA 2 (nhaA2), partial CSB-YP803478FGB
CSB-EP803478FGB
CSB-BP803478FGB
CSB-MP803478FGB
CSB-EP803478FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0324 membrane protein PP_3661 (PP_3661), partial CSB-YP803479FGB
CSB-EP803479FGB
CSB-BP803479FGB
CSB-MP803479FGB
CSB-EP803479FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable malate:quinone oxidoreductase 3 (mqo3), partial CSB-YP803480FGB
CSB-EP803480FGB
CSB-BP803480FGB
CSB-MP803480FGB
CSB-EP803480FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Acyl-homoserine lactone acylase pvdQ (pvdQ), partial CSB-YP803481FGB
CSB-EP803481FGB
CSB-BP803481FGB
CSB-MP803481FGB
CSB-EP803481FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urease accessory protein UreE (ureE) CSB-YP803482FGB
CSB-EP803482FGB
CSB-BP803482FGB
CSB-MP803482FGB
CSB-EP803482FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP803483FGB
CSB-EP803483FGB
CSB-BP803483FGB
CSB-MP803483FGB
CSB-EP803483FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L35 (rpmI) CSB-YP803484FGB
CSB-EP803484FGB
CSB-BP803484FGB
CSB-MP803484FGB
CSB-EP803484FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0502 protein PP_2442 (PP_2442) CSB-YP803485FGB
CSB-EP803485FGB
CSB-BP803485FGB
CSB-MP803485FGB
CSB-EP803485FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transcription elongation factor GreB (greB) CSB-YP803486FGB
CSB-EP803486FGB
CSB-BP803486FGB
CSB-MP803486FGB
CSB-EP803486FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transaldolase (tal) CSB-YP803487FGB
CSB-EP803487FGB
CSB-BP803487FGB
CSB-MP803487FGB
CSB-EP803487FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP803488FGB
CSB-EP803488FGB
CSB-BP803488FGB
CSB-MP803488FGB
CSB-EP803488FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Bifunctional protein FolD 1 (folD1) CSB-YP803489FGB
CSB-EP803489FGB
CSB-BP803489FGB
CSB-MP803489FGB
CSB-EP803489FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP803490FGB
CSB-EP803490FGB
CSB-BP803490FGB
CSB-MP803490FGB
CSB-EP803490FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cytidylate kinase (cmk) CSB-YP803491FGB
CSB-EP803491FGB
CSB-BP803491FGB
CSB-MP803491FGB
CSB-EP803491FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable septum site-determining protein MinC (minC) CSB-YP803492FGB
CSB-EP803492FGB
CSB-BP803492FGB
CSB-MP803492FGB
CSB-EP803492FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP803493FGB
CSB-EP803493FGB
CSB-BP803493FGB
CSB-MP803493FGB
CSB-EP803493FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA mismatch repair protein MutS (mutS), partial CSB-YP803494FGB
CSB-EP803494FGB
CSB-BP803494FGB
CSB-MP803494FGB
CSB-EP803494FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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