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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP811203LOS
CSB-EP811203LOS
CSB-BP811203LOS
CSB-MP811203LOS
CSB-EP811203LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP811204LOS
CSB-EP811204LOS
CSB-BP811204LOS
CSB-MP811204LOS
CSB-EP811204LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Macro domain-containing protein LA_4133 (LA_4133) CSB-YP811205LOS
CSB-EP811205LOS
CSB-BP811205LOS
CSB-MP811205LOS
CSB-EP811205LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 7-carboxy-7-deazaguanine synthase (queE) CSB-YP811206LOS
CSB-EP811206LOS
CSB-BP811206LOS
CSB-MP811206LOS
CSB-EP811206LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Methionine--tRNA ligase (metG), partial CSB-YP811207LOS
CSB-EP811207LOS
CSB-BP811207LOS
CSB-MP811207LOS
CSB-EP811207LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP811208LOS
CSB-EP811208LOS
CSB-BP811208LOS
CSB-MP811208LOS
CSB-EP811208LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Single-stranded DNA-binding protein (ssb) CSB-YP811209LOS
CSB-EP811209LOS
CSB-BP811209LOS
CSB-MP811209LOS
CSB-EP811209LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 50S ribosomal protein L10 (rplJ) CSB-YP811210LOS
CSB-EP811210LOS
CSB-BP811210LOS
CSB-MP811210LOS
CSB-EP811210LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Alanine--tRNA ligase (alaS), partial CSB-YP811211LOS
CSB-EP811211LOS
CSB-BP811211LOS
CSB-MP811211LOS
CSB-EP811211LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP811212LOS
CSB-EP811212LOS
CSB-BP811212LOS
CSB-MP811212LOS
CSB-EP811212LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai DNA translocase FtsK (ftsK), partial CSB-YP811213LOS
CSB-EP811213LOS
CSB-BP811213LOS
CSB-MP811213LOS
CSB-EP811213LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP811214LOS
CSB-EP811214LOS
CSB-BP811214LOS
CSB-MP811214LOS
CSB-EP811214LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Trigger factor (tig) CSB-YP811215LOS
CSB-EP811215LOS
CSB-BP811215LOS
CSB-MP811215LOS
CSB-EP811215LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Probable chemoreceptor glutamine deamidase CheD 1 (cheD1) CSB-YP811216LOS
CSB-EP811216LOS
CSB-BP811216LOS
CSB-MP811216LOS
CSB-EP811216LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Ribosome maturation factor RimM (rimM) CSB-YP811217LOS
CSB-EP811217LOS
CSB-BP811217LOS
CSB-MP811217LOS
CSB-EP811217LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai ATP-dependent protease subunit HslV (hslV) CSB-YP811218LOS
CSB-EP811218LOS
CSB-BP811218LOS
CSB-MP811218LOS
CSB-EP811218LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP811219LOS
CSB-EP811219LOS
CSB-BP811219LOS
CSB-MP811219LOS
CSB-EP811219LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai UvrABC system protein A (uvrA), partial CSB-YP811220LOS
CSB-EP811220LOS
CSB-BP811220LOS
CSB-MP811220LOS
CSB-EP811220LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Malate dehydrogenase (mdh) CSB-YP811221LOS
CSB-EP811221LOS
CSB-BP811221LOS
CSB-MP811221LOS
CSB-EP811221LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai D-alanine--D-alanine ligase (ddl) CSB-YP811222LOS
CSB-EP811222LOS
CSB-BP811222LOS
CSB-MP811222LOS
CSB-EP811222LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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