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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma penetrans Probable phosphopantetheine adenylyltransferase (coaD) CSB-YP811183MLU
CSB-EP811183MLU
CSB-BP811183MLU
CSB-MP811183MLU
CSB-EP811183MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP811184MLU
CSB-EP811184MLU
CSB-BP811184MLU
CSB-MP811184MLU
CSB-EP811184MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans UvrABC system protein A (uvrA), partial CSB-YP811185MLU
CSB-EP811185MLU
CSB-BP811185MLU
CSB-MP811185MLU
CSB-EP811185MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Lysine--tRNA ligase (lysS) CSB-YP811186MLU
CSB-EP811186MLU
CSB-BP811186MLU
CSB-MP811186MLU
CSB-EP811186MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP811187MLU
CSB-EP811187MLU
CSB-BP811187MLU
CSB-MP811187MLU
CSB-EP811187MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Bifunctional protein FolD (folD) CSB-YP811188MLU
CSB-EP811188MLU
CSB-BP811188MLU
CSB-MP811188MLU
CSB-EP811188MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans 50S ribosomal protein L10 (rplJ) CSB-YP811189MLU
CSB-EP811189MLU
CSB-BP811189MLU
CSB-MP811189MLU
CSB-EP811189MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans UPF0122 protein MYPE4850 (MYPE4850) CSB-YP811190MLU
CSB-EP811190MLU
CSB-BP811190MLU
CSB-MP811190MLU
CSB-EP811190MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Chaperone protein ClpB (clpB), partial CSB-YP811191MLU
CSB-EP811191MLU
CSB-BP811191MLU
CSB-MP811191MLU
CSB-EP811191MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP811192MLU
CSB-EP811192MLU
CSB-BP811192MLU
CSB-MP811192MLU
CSB-EP811192MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP811193MLU
CSB-EP811193MLU
CSB-BP811193MLU
CSB-MP811193MLU
CSB-EP811193MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans GTPase obg (obg) CSB-YP811194MLU
CSB-EP811194MLU
CSB-BP811194MLU
CSB-MP811194MLU
CSB-EP811194MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP811195MLU
CSB-EP811195MLU
CSB-BP811195MLU
CSB-MP811195MLU
CSB-EP811195MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP811196MLU
CSB-EP811196MLU
CSB-BP811196MLU
CSB-MP811196MLU
CSB-EP811196MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Translation initiation factor IF-2 (infB), partial CSB-YP811197MLU
CSB-EP811197MLU
CSB-BP811197MLU
CSB-MP811197MLU
CSB-EP811197MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans ATP synthase subunit beta (atpD), partial CSB-YP811198MLU
CSB-EP811198MLU
CSB-BP811198MLU
CSB-MP811198MLU
CSB-EP811198MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma penetrans Probable GTP-binding protein EngB (engB) CSB-YP811199MLU
CSB-EP811199MLU
CSB-BP811199MLU
CSB-MP811199MLU
CSB-EP811199MLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Alanine racemase (alr) CSB-YP811200LOS
CSB-EP811200LOS
CSB-BP811200LOS
CSB-MP811200LOS
CSB-EP811200LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP811201LOS
CSB-EP811201LOS
CSB-BP811201LOS
CSB-MP811201LOS
CSB-EP811201LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Adenosylcobalamin biosynthesis bifunctional protein CobDQ (cobDQ), partial CSB-YP811202LOS
CSB-EP811202LOS
CSB-BP811202LOS
CSB-MP811202LOS
CSB-EP811202LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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