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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0324 membrane protein SAG1125 (SAG1125), partial CSB-YP812267SMI
CSB-EP812267SMI
CSB-BP812267SMI
CSB-MP812267SMI
CSB-EP812267SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable potassium transport system protein kup (kup), partial CSB-YP812268SMI
CSB-EP812268SMI
CSB-BP812268SMI
CSB-MP812268SMI
CSB-EP812268SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0223 protein SAG0995 (SAG0995) CSB-YP812269SMI
CSB-EP812269SMI
CSB-BP812269SMI
CSB-MP812269SMI
CSB-EP812269SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP812270SMI
CSB-EP812270SMI
CSB-BP812270SMI
CSB-MP812270SMI
CSB-EP812270SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP812271SMI
CSB-EP812271SMI
CSB-BP812271SMI
CSB-MP812271SMI
CSB-EP812271SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP812272SMI
CSB-EP812272SMI
CSB-BP812272SMI
CSB-MP812272SMI
CSB-EP812272SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP812273SMI
CSB-EP812273SMI
CSB-BP812273SMI
CSB-MP812273SMI
CSB-EP812273SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0213 protein SAG0778 (SAG0778) CSB-YP812274SMI
CSB-EP812274SMI
CSB-BP812274SMI
CSB-MP812274SMI
CSB-EP812274SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP812275SMI
CSB-EP812275SMI
CSB-BP812275SMI
CSB-MP812275SMI
CSB-EP812275SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine--tRNA ligase (thrS), partial CSB-YP812276SMI
CSB-EP812276SMI
CSB-BP812276SMI
CSB-MP812276SMI
CSB-EP812276SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP812277SMI
CSB-EP812277SMI
CSB-BP812277SMI
CSB-MP812277SMI
CSB-EP812277SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP812278SMI
CSB-EP812278SMI
CSB-BP812278SMI
CSB-MP812278SMI
CSB-EP812278SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell cycle protein GpsB (gpsB) CSB-YP812279SMI
CSB-EP812279SMI
CSB-BP812279SMI
CSB-MP812279SMI
CSB-EP812279SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FMN-dependent NADH-azoreductase (azoR) CSB-YP812280SMI
CSB-EP812280SMI
CSB-BP812280SMI
CSB-MP812280SMI
CSB-EP812280SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP812281SMI
CSB-EP812281SMI
CSB-BP812281SMI
CSB-MP812281SMI
CSB-EP812281SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP812282SMI1
CSB-EP812282SMI1
CSB-BP812282SMI1
CSB-MP812282SMI1
CSB-EP812282SMI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP812283SMI
CSB-EP812283SMI
CSB-BP812283SMI
CSB-MP812283SMI
CSB-EP812283SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP812284SMI
CSB-EP812284SMI
CSB-BP812284SMI
CSB-MP812284SMI
CSB-EP812284SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP812285SMI
CSB-EP812285SMI
CSB-BP812285SMI
CSB-MP812285SMI
CSB-EP812285SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP812286SMI
CSB-EP812286SMI
CSB-BP812286SMI
CSB-MP812286SMI
CSB-EP812286SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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