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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae UPF0042 nucleotide-binding protein spr1424 (spr1424) CSB-YP812187SMP
CSB-EP812187SMP
CSB-BP812187SMP
CSB-MP812187SMP
CSB-EP812187SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0246 protein spr1405 (spr1405) CSB-YP812188SMP
CSB-EP812188SMP
CSB-BP812188SMP
CSB-MP812188SMP
CSB-EP812188SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Peptide deformylase (def) CSB-YP812189SMP
CSB-EP812189SMP
CSB-BP812189SMP
CSB-MP812189SMP
CSB-EP812189SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 3-dehydroquinate synthase (aroB) CSB-YP812190SMP
CSB-EP812190SMP
CSB-BP812190SMP
CSB-MP812190SMP
CSB-EP812190SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Protease HtpX homolog (htpX), partial CSB-YP812191SMP1
CSB-EP812191SMP1
CSB-BP812191SMP1
CSB-MP812191SMP1
CSB-EP812191SMP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP812192SMP
CSB-EP812192SMP
CSB-BP812192SMP
CSB-MP812192SMP
CSB-EP812192SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Tagatose 1,6-diphosphate aldolase (lacD) CSB-YP812193SMP
CSB-EP812193SMP
CSB-BP812193SMP
CSB-MP812193SMP
CSB-EP812193SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP812194SMP
CSB-EP812194SMP
CSB-BP812194SMP
CSB-MP812194SMP
CSB-EP812194SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae DNA ligase (ligA), partial CSB-YP812195SMP
CSB-EP812195SMP
CSB-BP812195SMP
CSB-MP812195SMP
CSB-EP812195SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP812196SMP
CSB-EP812196SMP
CSB-BP812196SMP
CSB-MP812196SMP
CSB-EP812196SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Peptide chain release factor 1 (prfA) CSB-YP812197SMP
CSB-EP812197SMP
CSB-BP812197SMP
CSB-MP812197SMP
CSB-EP812197SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Foldase protein prsA (prsA) CSB-YP812198SMP
CSB-EP812198SMP
CSB-BP812198SMP
CSB-MP812198SMP
CSB-EP812198SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP812199SMP
CSB-EP812199SMP
CSB-BP812199SMP
CSB-MP812199SMP
CSB-EP812199SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Gamma-glutamyl phosphate reductase (proA) CSB-YP812200SMP
CSB-EP812200SMP
CSB-BP812200SMP
CSB-MP812200SMP
CSB-EP812200SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Deoxyribose-phosphate aldolase (deoC) CSB-YP812201SMP
CSB-EP812201SMP
CSB-BP812201SMP
CSB-MP812201SMP
CSB-EP812201SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribosome maturation factor RimM (rimM) CSB-YP812202SMP
CSB-EP812202SMP
CSB-BP812202SMP
CSB-MP812202SMP
CSB-EP812202SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Uracil phosphoribosyltransferase (upp) CSB-YP812203SMP
CSB-EP812203SMP
CSB-BP812203SMP
CSB-MP812203SMP
CSB-EP812203SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP812204SMP
CSB-EP812204SMP
CSB-BP812204SMP
CSB-MP812204SMP
CSB-EP812204SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Mini-ribonuclease 3 (mrnC) CSB-YP812205SMP
CSB-EP812205SMP
CSB-BP812205SMP
CSB-MP812205SMP
CSB-EP812205SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Valine--tRNA ligase (valS), partial CSB-YP812206SMP
CSB-EP812206SMP
CSB-BP812206SMP
CSB-MP812206SMP
CSB-EP812206SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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