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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Putative fatty acid elongation protein 3 (elo-3), partial CSB-YP344180CXY1
CSB-EP344180CXY1
CSB-BP344180CXY1
CSB-MP344180CXY1
CSB-EP344180CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L32-1 (RPL32A) CSB-YP344181DOA
CSB-EP344181DOA
CSB-BP344181DOA
CSB-MP344181DOA
CSB-EP344181DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Melatonin receptor type 1C (mtnr1c), partial CSB-YP344182XBE1
CSB-EP344182XBE1
CSB-BP344182XBE1
CSB-MP344182XBE1
CSB-EP344182XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus radiodurans 50S ribosomal protein L32 (rpmF) CSB-YP344183DJA
CSB-EP344183DJA
CSB-BP344183DJA
CSB-MP344183DJA
CSB-EP344183DJA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica campestris Glucan endo-1,3-beta-glucosidase (BGL) CSB-YP344184BWB
CSB-EP344184BWB
CSB-BP344184BWB
CSB-MP344184BWB
CSB-EP344184BWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L35 (rpmI) CSB-YP344185BXE
CSB-EP344185BXE
CSB-BP344185BXE
CSB-MP344185BXE
CSB-EP344185BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays IN2-2 protein (IN2-2) CSB-YP344186ZAX
CSB-EP344186ZAX
CSB-BP344186ZAX
CSB-MP344186ZAX
CSB-EP344186ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apomastus schlingeri U3-cyrtautoxin-As1a CSB-YP344187AMR
CSB-EP344187AMR
CSB-BP344187AMR
CSB-MP344187AMR
CSB-EP344187AMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein Malvolio (Mvl), partial CSB-YP344188DLU
CSB-EP344188DLU
CSB-BP344188DLU
CSB-MP344188DLU
CSB-EP344188DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Melatonin receptor type 1C, partial CSB-YP344189CH1
CSB-EP344189CH1
CSB-BP344189CH1
CSB-MP344189CH1
CSB-EP344189CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Isocitrate lyase, partial CSB-YP344190CXP
CSB-EP344190CXP
CSB-BP344190CXP
CSB-MP344190CXP
CSB-EP344190CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Rhizopine catabolism protein mocC (mocC) CSB-YP344191RKT
CSB-EP344191RKT
CSB-BP344191RKT
CSB-MP344191RKT
CSB-EP344191RKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Glycine-rich RNA-binding protein GRP1A CSB-YP344192SZO
CSB-EP344192SZO
CSB-BP344192SZO
CSB-MP344192SZO
CSB-EP344192SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Non-heme chloroperoxidase (cpo) CSB-YP344193FOI
CSB-EP344193FOI
CSB-BP344193FOI
CSB-MP344193FOI
CSB-EP344193FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Probable glutathione S-transferase parC (PARC) CSB-YP344194NHE
CSB-EP344194NHE
CSB-BP344194NHE
CSB-MP344194NHE
CSB-EP344194NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine cleavage system H protein, mitochondrial (GDCSH) CSB-YP344195FBQ
CSB-EP344195FBQ
CSB-BP344195FBQ
CSB-MP344195FBQ
CSB-EP344195FBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis NAD (P)H-dependent D-xylose reductase CSB-YP344196KBK
CSB-EP344196KBK
CSB-BP344196KBK
CSB-MP344196KBK
CSB-EP344196KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus 60S ribosomal protein L16, mitochondrial (RPL16) CSB-YP344197BWD
CSB-EP344197BWD
CSB-BP344197BWD
CSB-MP344197BWD
CSB-EP344197BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio anguillarum Chloramphenicol acetyltransferase (cat) CSB-YP344198VEV
CSB-EP344198VEV
CSB-BP344198VEV
CSB-MP344198VEV
CSB-EP344198VEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontella sinensis DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP344199OAK
CSB-EP344199OAK
CSB-BP344199OAK
CSB-MP344199OAK
CSB-EP344199OAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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