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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uncharacterized protein Mb1353c (Mb1353c), partial CSB-YP363627MVH1
CSB-EP363627MVH1
CSB-BP363627MVH1
CSB-MP363627MVH1
CSB-EP363627MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycogen operon protein GlgX homolog (glgX), partial CSB-YP363628MVH
CSB-EP363628MVH
CSB-BP363628MVH
CSB-MP363628MVH
CSB-EP363628MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylglutamate kinase (argB) CSB-YP363629MVH
CSB-EP363629MVH
CSB-BP363629MVH
CSB-MP363629MVH
CSB-EP363629MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR) CSB-YP363630MVH
CSB-EP363630MVH
CSB-BP363630MVH
CSB-MP363630MVH
CSB-EP363630MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase (aroK) CSB-YP363631MVH
CSB-EP363631MVH
CSB-BP363631MVH
CSB-MP363631MVH
CSB-EP363631MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate synthase (aroB) CSB-YP363632MVZ
CSB-EP363632MVZ
CSB-BP363632MVZ
CSB-MP363632MVZ
CSB-EP363632MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP363633MVH
CSB-EP363633MVH
CSB-BP363633MVH
CSB-MP363633MVH
CSB-EP363633MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartokinase (ask) CSB-YP363634MVZ
CSB-EP363634MVZ
CSB-BP363634MVZ
CSB-MP363634MVZ
CSB-EP363634MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting P-type ATPase C (ctpC), partial CSB-YP363635MVZ1
CSB-EP363635MVZ1
CSB-BP363635MVZ1
CSB-MP363635MVZ1
CSB-EP363635MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting ATPase E (ctpE), partial CSB-YP363636MVZ
CSB-EP363636MVZ
CSB-BP363636MVZ
CSB-MP363636MVZ
CSB-EP363636MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Biotin synthase (bioB) CSB-YP363637MVZ
CSB-EP363637MVZ
CSB-BP363637MVZ
CSB-MP363637MVZ
CSB-EP363637MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-/propionyl-coenzyme A carboxylase alpha chain (accA1), partial CSB-YP363638MVH
CSB-EP363638MVH
CSB-BP363638MVH
CSB-MP363638MVH
CSB-EP363638MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Biotinylated protein TB7.3 (Rv3221c, MT3317) CSB-YP363639MVZ
CSB-EP363639MVZ
CSB-BP363639MVZ
CSB-MP363639MVZ
CSB-EP363639MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Chaperonin GroEL 1 (groEL1), partial CSB-YP363640MVZ
CSB-EP363640MVZ
CSB-BP363640MVZ
CSB-MP363640MVZ
CSB-EP363640MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin 2 (groL2), partial CSB-YP363641MVZ
CSB-EP363641MVZ
CSB-BP363641MVZ
CSB-MP363641MVZ
CSB-EP363641MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ATP-dependent Clp protease ATP-binding subunit (clpC), partial CSB-YP363642MVH
CSB-EP363642MVH
CSB-BP363642MVH
CSB-MP363642MVH
CSB-EP363642MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb2686 (Mb2686) CSB-YP363643MVH
CSB-EP363643MVH
CSB-BP363643MVH
CSB-MP363643MVH
CSB-EP363643MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP363644MVH
CSB-EP363644MVH
CSB-BP363644MVH
CSB-MP363644MVH
CSB-EP363644MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP363645MVZ
CSB-EP363645MVZ
CSB-BP363645MVZ
CSB-MP363645MVZ
CSB-EP363645MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopantetheine adenylyltransferase (coaD) CSB-YP363646MVZ
CSB-EP363646MVZ
CSB-BP363646MVZ
CSB-MP363646MVZ
CSB-EP363646MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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