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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Clathrin coat assembly protein AP180B (YAP1802), partial CSB-YP345277SVG
CSB-EP345277SVG
CSB-BP345277SVG
CSB-MP345277SVG
CSB-EP345277SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR251W (YGR251W) CSB-YP345278SVG
CSB-EP345278SVG
CSB-BP345278SVG
CSB-MP345278SVG
CSB-EP345278SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Steryl acetyl hydrolase 1 (SAY1), partial CSB-YP345279SVG
CSB-EP345279SVG
CSB-BP345279SVG
CSB-MP345279SVG
CSB-EP345279SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized membrane protein YGR290W (YGR290W), partial CSB-YP345280SVG1
CSB-EP345280SVG1
CSB-BP345280SVG1
CSB-MP345280SVG1
CSB-EP345280SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Tyrosine-protein kinase HTK16 (HTK16), partial CSB-YP345281HXN
CSB-EP345281HXN
CSB-BP345281HXN
CSB-MP345281HXN
CSB-EP345281HXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mrp homolog (mrp) CSB-YP345282MVN
CSB-EP345282MVN
CSB-BP345282MVN
CSB-MP345282MVN
CSB-EP345282MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein HOL1 (HOL1), partial CSB-YP345283SVG
CSB-EP345283SVG
CSB-BP345283SVG
CSB-MP345283SVG
CSB-EP345283SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Glucose transporter type 3 (Glut3), partial CSB-YP345284DLU
CSB-EP345284DLU
CSB-BP345284DLU
CSB-MP345284DLU
CSB-EP345284DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus tschawytscha Insulin gene enhancer protein ISL-2A (isl2a) CSB-YP345285OBK
CSB-EP345285OBK
CSB-BP345285OBK
CSB-MP345285OBK
CSB-EP345285OBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP345286MVN
CSB-EP345286MVN
CSB-BP345286MVN
CSB-MP345286MVN
CSB-EP345286MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) CSB-YP345287GFW
CSB-EP345287GFW
CSB-BP345287GFW
CSB-MP345287GFW
CSB-EP345287GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Low molecular weight protein-tyrosine-phosphatase (ptpA) CSB-YP345288FOB
CSB-EP345288FOB
CSB-BP345288FOB
CSB-MP345288FOB
CSB-EP345288FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lampetra japonica Fructose-bisphosphate aldolase, non-muscle type CSB-YP345289LCB
CSB-EP345289LCB
CSB-BP345289LCB
CSB-MP345289LCB
CSB-EP345289LCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diphyllobothrium dendriticum Actin-2 (ACT2) CSB-YP345290DEN
CSB-EP345290DEN
CSB-BP345290DEN
CSB-MP345290DEN
CSB-EP345290DEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halocynthia roretzi Actin, muscle 1A (MA1A) CSB-YP345291HTQ
CSB-EP345291HTQ
CSB-BP345291HTQ
CSB-MP345291HTQ
CSB-EP345291HTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molgula oculata Actin, larval muscle-type CSB-YP345292MHG
CSB-EP345292MHG
CSB-BP345292MHG
CSB-MP345292MHG
CSB-EP345292MHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Actin-2 CSB-YP345293SXQ
CSB-EP345293SXQ
CSB-BP345293SXQ
CSB-MP345293SXQ
CSB-EP345293SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer membrane usher protein CssD (cssD) CSB-YP345294ENL
CSB-EP345294ENL
CSB-BP345294ENL
CSB-MP345294ENL
CSB-EP345294ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cob (I)yrinic acid a,c-diamide adenosyltransferase CSB-YP345295MVN
CSB-EP345295MVN
CSB-BP345295MVN
CSB-MP345295MVN
CSB-EP345295MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clarias gariepinus Gonadotropin subunit beta-2 (cgbb) CSB-YP345296DTT
CSB-EP345296DTT
CSB-BP345296DTT
CSB-MP345296DTT
CSB-EP345296DTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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