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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Beta-lactamase (blaB) CSB-YP345620EZA
CSB-EP345620EZA
CSB-BP345620EZA
CSB-MP345620EZA
CSB-EP345620EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis HTH-type transcriptional regulator ttuA (ttuA) CSB-YP345621AYT
CSB-EP345621AYT
CSB-BP345621AYT
CSB-MP345621AYT
CSB-EP345621AYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PCP degradation transcriptional activation protein (pcpR) CSB-YP345622SJK
CSB-EP345622SJK
CSB-BP345622SJK
CSB-MP345622SJK
CSB-EP345622SJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HU-beta (hupB) CSB-YP345623SYN
CSB-EP345623SYN
CSB-BP345623SYN
CSB-MP345623SYN
CSB-EP345623SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Transcriptional activator protein nhaR (nhaR) CSB-YP345624VEX
CSB-EP345624VEX
CSB-BP345624VEX
CSB-MP345624VEX
CSB-EP345624VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia solanacearum HTH-type transcriptional regulator phcA (phcA) CSB-YP345625RAR
CSB-EP345625RAR
CSB-BP345625RAR
CSB-MP345625RAR
CSB-EP345625RAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine-type carboxypeptidase F (pepF) CSB-YP345626DOZ
CSB-EP345626DOZ
CSB-BP345626DOZ
CSB-MP345626DOZ
CSB-EP345626DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zoarces viviparus Metallothionein (mt) CSB-YP345627ZAJ
CSB-EP345627ZAJ
CSB-BP345627ZAJ
CSB-MP345627ZAJ
CSB-EP345627ZAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisolithus tinctorius Hydrophobin-2 (HYD-2) CSB-YP345628PKX
CSB-EP345628PKX
CSB-BP345628PKX
CSB-MP345628PKX
CSB-EP345628PKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrophobin-like protein ssgA (SSGA) CSB-YP345629MRL
CSB-EP345629MRL
CSB-BP345629MRL
CSB-MP345629MRL
CSB-EP345629MRL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus sylvestris Glutamine synthetase cytosolic isozyme CSB-YP345630PYS
CSB-EP345630PYS
CSB-BP345630PYS
CSB-MP345630PYS
CSB-EP345630PYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Indoleacetamide hydrolase (iaaH) CSB-YP345631PWD
CSB-EP345631PWD
CSB-BP345631PWD
CSB-MP345631PWD
CSB-EP345631PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio proteolyticus 30S ribosomal protein S5 (rpsE) CSB-YP345632VCN
CSB-EP345632VCN
CSB-BP345632VCN
CSB-MP345632VCN
CSB-EP345632VCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Phosphoserine aminotransferase, chloroplastic CSB-YP345633FKI
CSB-EP345633FKI
CSB-BP345633FKI
CSB-MP345633FKI
CSB-EP345633FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription factor atf1 (atf1), partial CSB-YP345634SXV
CSB-EP345634SXV
CSB-BP345634SXV
CSB-MP345634SXV
CSB-EP345634SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis macroura Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (PDHA) CSB-YP345635SZU
CSB-EP345635SZU
CSB-BP345635SZU
CSB-MP345635SZU
CSB-EP345635SZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative nucleoside-triphosphatase (NTP4), partial CSB-YP345636TOV
CSB-EP345636TOV
CSB-BP345636TOV
CSB-MP345636TOV
CSB-EP345636TOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stage 0 sporulation protein A (spo0A) CSB-YP345637BWM
CSB-EP345637BWM
CSB-BP345637BWM
CSB-MP345637BWM
CSB-EP345637BWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stage 0 sporulation protein A homolog (spo0A) CSB-YP345638DUK
CSB-EP345638DUK
CSB-BP345638DUK
CSB-MP345638DUK
CSB-EP345638DUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Glutamate racemase (murI) CSB-YP345639HTA
CSB-EP345639HTA
CSB-BP345639HTA
CSB-MP345639HTA
CSB-EP345639HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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