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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Probable family 17 glucosidase SCW11 (SCW11), partial CSB-YP347389SVG
CSB-EP347389SVG
CSB-BP347389SVG
CSB-MP347389SVG
CSB-EP347389SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1 (CDH1), partial CSB-YP347390SVG
CSB-EP347390SVG
CSB-BP347390SVG
CSB-MP347390SVG
CSB-EP347390SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR017W (YGR017W) CSB-YP347391SVG
CSB-EP347391SVG
CSB-BP347391SVG
CSB-MP347391SVG
CSB-EP347391SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR025W (YGR025W) CSB-YP347392SVG
CSB-EP347392SVG
CSB-BP347392SVG
CSB-MP347392SVG
CSB-EP347392SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dolichyldiphosphatase (CAX4), partial CSB-YP347393SVG1
CSB-EP347393SVG1
CSB-BP347393SVG1
CSB-MP347393SVG1
CSB-EP347393SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR050C (YGR050C) CSB-YP347394SVG
CSB-EP347394SVG
CSB-BP347394SVG
CSB-MP347394SVG
CSB-EP347394SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR079W (YGR079W) CSB-YP347395SVG
CSB-EP347395SVG
CSB-BP347395SVG
CSB-MP347395SVG
CSB-EP347395SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U3 small nucleolar RNA-associated protein 22 (UTP22), partial CSB-YP347396SVG
CSB-EP347396SVG
CSB-BP347396SVG
CSB-MP347396SVG
CSB-EP347396SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glutamyl-tRNA (Gln) amidotransferase subunit F, mitochondrial, partial CSB-YP347397SVG
CSB-EP347397SVG
CSB-BP347397SVG
CSB-MP347397SVG
CSB-EP347397SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR115C (YGR115C), partial CSB-YP347398SVG1
CSB-EP347398SVG1
CSB-BP347398SVG1
CSB-MP347398SVG1
CSB-EP347398SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U3 small nucleolar RNA-associated protein 8 (UTP8), partial CSB-YP347399SVG
CSB-EP347399SVG
CSB-BP347399SVG
CSB-MP347399SVG
CSB-EP347399SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 62 (VPS62), partial CSB-YP347400SVG1
CSB-EP347400SVG1
CSB-BP347400SVG1
CSB-MP347400SVG1
CSB-EP347400SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein phosphatase type 2A regulatory subunit RTS3 (RTS3) CSB-YP347401SVG
CSB-EP347401SVG
CSB-BP347401SVG
CSB-MP347401SVG
CSB-EP347401SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alcohol O-acetyltransferase 2 (ATF2), partial CSB-YP347402SVG
CSB-EP347402SVG
CSB-BP347402SVG
CSB-MP347402SVG
CSB-EP347402SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR190C (YGR190C) CSB-YP347403SVG
CSB-EP347403SVG
CSB-BP347403SVG
CSB-MP347403SVG
CSB-EP347403SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 6-phosphogluconolactonase 4 (SOL4) CSB-YP347404SVG
CSB-EP347404SVG
CSB-BP347404SVG
CSB-MP347404SVG
CSB-EP347404SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR265W (YGR265W), partial CSB-YP347405SVG1
CSB-EP347405SVG1
CSB-BP347405SVG1
CSB-MP347405SVG1
CSB-EP347405SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 102 (RTT102) CSB-YP347406SVG
CSB-EP347406SVG
CSB-BP347406SVG
CSB-MP347406SVG
CSB-EP347406SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein PXR1 (PXR1) CSB-YP347407SVG
CSB-EP347407SVG
CSB-BP347407SVG
CSB-MP347407SVG
CSB-EP347407SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR291C (YGR291C) CSB-YP347408SVG
CSB-EP347408SVG
CSB-BP347408SVG
CSB-MP347408SVG
CSB-EP347408SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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