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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neisseria meningitidis serogroup A / serotype 4A 60 kDa chaperonin (groL), partial CSB-YP347899NGF
CSB-EP347899NGF
CSB-BP347899NGF
CSB-MP347899NGF
CSB-EP347899NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Outer-membrane lipoprotein LolB (lolB) CSB-YP347900NGG
CSB-EP347900NGG
CSB-BP347900NGG
CSB-MP347900NGG
CSB-EP347900NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP347901XBL
CSB-EP347901XBL
CSB-BP347901XBL
CSB-MP347901XBL
CSB-EP347901XBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Glucose-1-phosphate thymidylyltransferase (rmlA1) CSB-YP347902NGF
CSB-EP347902NGF
CSB-BP347902NGF
CSB-MP347902NGF
CSB-EP347902NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Outer-membrane lipoprotein carrier protein (lolA) CSB-YP347903NGG
CSB-EP347903NGG
CSB-BP347903NGG
CSB-MP347903NGG
CSB-EP347903NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 30S ribosomal protein S21 (rpsU) CSB-YP347904MLN
CSB-EP347904MLN
CSB-BP347904MLN
CSB-MP347904MLN
CSB-EP347904MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP synthase subunit c (atpE), partial CSB-YP347905BWZ1
CSB-EP347905BWZ1
CSB-BP347905BWZ1
CSB-MP347905BWZ1
CSB-EP347905BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum DNA polymerase III subunit beta (dnaN) CSB-YP347906BWZ
CSB-EP347906BWZ
CSB-BP347906BWZ
CSB-MP347906BWZ
CSB-EP347906BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Primosomal protein 1 (dnaT) CSB-YP347907BWZ
CSB-EP347907BWZ
CSB-BP347907BWZ
CSB-MP347907BWZ
CSB-EP347907BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum DNA-binding protein HU (hup) CSB-YP347908BWZ
CSB-EP347908BWZ
CSB-BP347908BWZ
CSB-MP347908BWZ
CSB-EP347908BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Preprotein translocase subunit SecE (secE), partial CSB-YP347909BWZ1
CSB-EP347909BWZ1
CSB-BP347909BWZ1
CSB-MP347909BWZ1
CSB-EP347909BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Uncharacterized protein BU052 (BU052) CSB-YP347910BWZ
CSB-EP347910BWZ
CSB-BP347910BWZ
CSB-MP347910BWZ
CSB-EP347910BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Uncharacterized sugar kinase BU060 (BU060) CSB-YP347911BWZ
CSB-EP347911BWZ
CSB-BP347911BWZ
CSB-MP347911BWZ
CSB-EP347911BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Flagellar motor switch protein FliG (fliG) CSB-YP347912BWZ
CSB-EP347912BWZ
CSB-BP347912BWZ
CSB-MP347912BWZ
CSB-EP347912BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Flagellar biosynthetic protein fliP (fliP), partial CSB-YP347913BWZ1
CSB-EP347913BWZ1
CSB-BP347913BWZ1
CSB-MP347913BWZ1
CSB-EP347913BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP347914BWZ
CSB-EP347914BWZ
CSB-BP347914BWZ
CSB-MP347914BWZ
CSB-EP347914BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP phosphoribosyltransferase (hisG) CSB-YP347915BWZ
CSB-EP347915BWZ
CSB-BP347915BWZ
CSB-MP347915BWZ
CSB-EP347915BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP347916BWZ
CSB-EP347916BWZ
CSB-BP347916BWZ
CSB-MP347916BWZ
CSB-EP347916BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Electron transport complex protein RnfD (rnfD), partial CSB-YP347917BWZ
CSB-EP347917BWZ
CSB-BP347917BWZ
CSB-MP347917BWZ
CSB-EP347917BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH) CSB-YP347918BWZ
CSB-EP347918BWZ
CSB-BP347918BWZ
CSB-MP347918BWZ
CSB-EP347918BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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