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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Diaminopimelate epimerase (dapF) CSB-YP351938MVH
CSB-EP351938MVH
CSB-BP351938MVH
CSB-MP351938MVH
CSB-EP351938MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Deoxycytidine triphosphate deaminase (dcd) CSB-YP351939NGG
CSB-EP351939NGG
CSB-BP351939NGG
CSB-MP351939NGG
CSB-EP351939NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Peptide deformylase-like (BMEII0812) CSB-YP351940BMO
CSB-EP351940BMO
CSB-BP351940BMO
CSB-MP351940BMO
CSB-EP351940BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide deformylase-like (SA1058) CSB-YP351941SKY
CSB-EP351941SKY
CSB-BP351941SKY
CSB-MP351941SKY
CSB-EP351941SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopentomutase (deoB) CSB-YP351942SWW
CSB-EP351942SWW
CSB-BP351942SWW
CSB-MP351942SWW
CSB-EP351942SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopentomutase (deoB) CSB-YP351943SMT
CSB-EP351943SMT
CSB-BP351943SMT
CSB-MP351943SMT
CSB-EP351943SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyribose-phosphate aldolase (deoC) CSB-YP351944MVH
CSB-EP351944MVH
CSB-BP351944MVH
CSB-MP351944MVH
CSB-EP351944MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 3-hydroxyisobutyrate dehydrogenase (mmsB) CSB-YP351945MVZ
CSB-EP351945MVZ
CSB-BP351945MVZ
CSB-MP351945MVZ
CSB-EP351945MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP351946EOD
CSB-EP351946EOD
CSB-BP351946EOD
CSB-MP351946EOD
CSB-EP351946EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrodipicolinate synthase (dapA) CSB-YP351947SKY
CSB-EP351947SKY
CSB-BP351947SKY
CSB-MP351947SKY
CSB-EP351947SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidinol dehydrogenase (hisD) CSB-YP351948SKX
CSB-EP351948SKX
CSB-BP351948SKX
CSB-MP351948SKX
CSB-EP351948SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP351949SUL
CSB-EP351949SUL
CSB-BP351949SUL
CSB-MP351949SUL
CSB-EP351949SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP351950SMP
CSB-EP351950SMP
CSB-BP351950SMP
CSB-MP351950SMP
CSB-EP351950SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA primase (dnaG), partial CSB-YP351951MVH
CSB-EP351951MVH
CSB-BP351951MVH
CSB-MP351951MVH
CSB-EP351951MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA primase (dnaG), partial CSB-YP351952SKY
CSB-EP351952SKY
CSB-BP351952SKY
CSB-MP351952SKY
CSB-EP351952SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Chaperone protein DnaJ (dnaJ) CSB-YP351953NGF
CSB-EP351953NGF
CSB-BP351953NGF
CSB-MP351953NGF
CSB-EP351953NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaJ (dnaJ) CSB-YP351954SKY
CSB-EP351954SKY
CSB-BP351954SKY
CSB-MP351954SKY
CSB-EP351954SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase III subunit gamma/tau (dnaX), partial CSB-YP351955MVZ
CSB-EP351955MVZ
CSB-BP351955MVZ
CSB-MP351955MVZ
CSB-EP351955MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase III subunit alpha (dnaE), partial CSB-YP351956MVH
CSB-EP351956MVH
CSB-BP351956MVH
CSB-MP351956MVH
CSB-EP351956MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit alpha (dnaE), partial CSB-YP351957SKY
CSB-EP351957SKY
CSB-BP351957SKY
CSB-MP351957SKY
CSB-EP351957SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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