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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli HTH-type transcriptional regulator galR (galR) CSB-YP355927ENV
CSB-EP355927ENV
CSB-BP355927ENV
CSB-MP355927ENV
CSB-EP355927ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional activator protein lysR (lysR) CSB-YP355928ENV
CSB-EP355928ENV
CSB-BP355928ENV
CSB-MP355928ENV
CSB-EP355928ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage lambda Repressor protein CI (CI) CSB-YP355929ECW
CSB-EP355929ECW
CSB-BP355929ECW
CSB-MP355929ECW
CSB-EP355929ECW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline repressor protein class C (tetR) CSB-YP355930ENL
CSB-EP355930ENL
CSB-BP355930ENL
CSB-MP355930ENL
CSB-EP355930ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P22 Transcriptional activator protein C1 (C1) CSB-YP355931EDG
CSB-EP355931EDG
CSB-BP355931EDG
CSB-MP355931EDG
CSB-EP355931EDG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage lambda Regulatory protein CIII (CIII) CSB-YP355932ECW
CSB-EP355932ECW
CSB-BP355932ECW
CSB-MP355932ECW
CSB-EP355932ECW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage lambda Antitermination protein N (N) CSB-YP355933ECW
CSB-EP355933ECW
CSB-BP355933ECW
CSB-MP355933ECW
CSB-EP355933ECW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SP01 RNA polymerase sigma GP28 factor (28) CSB-YP355934BQY
CSB-EP355934BQY
CSB-BP355934BQY
CSB-MP355934BQY
CSB-EP355934BQY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein rop (rop) CSB-YP355935ENL
CSB-EP355935ENL
CSB-BP355935ENL
CSB-MP355935ENL
CSB-EP355935ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Trp operon leader peptide (trpL) CSB-YP355936SXB
CSB-EP355936SXB
CSB-BP355936SXB
CSB-MP355936SXB
CSB-EP355936SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli IlvBN operon leader peptide (ivbL) CSB-YP355937ENV
CSB-EP355937ENV
CSB-BP355937ENV
CSB-MP355937ENV
CSB-EP355937ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repC) CSB-YP355938FKZ
CSB-EP355938FKZ
CSB-BP355938FKZ
CSB-MP355938FKZ
CSB-EP355938FKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repA) CSB-YP355939ENL
CSB-EP355939ENL
CSB-BP355939ENL
CSB-MP355939ENL
CSB-EP355939ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian virus 40 Large T antigen, partial CSB-YP355940SHP
CSB-EP355940SHP
CSB-BP355940SHP
CSB-MP355940SHP
CSB-EP355940SHP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Large T antigen, partial CSB-YP355941MKI
CSB-EP355941MKI
CSB-BP355941MKI
CSB-MP355941MKI
CSB-EP355941MKI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Middle T antigen, partial CSB-YP355942MKH
CSB-EP355942MKH
CSB-BP355942MKH
CSB-MP355942MKH
CSB-EP355942MKH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hamster polyomavirus Small t antigen CSB-YP355943HBZ
CSB-EP355943HBZ
CSB-BP355943HBZ
CSB-MP355943HBZ
CSB-EP355943HBZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant JC polyomavirus Small t antigen CSB-YP355944JAK
CSB-BP355944JAK
CSB-MP355944JAK
CSB-EP355944JAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant JC polyomavirus Agnoprotein, partial CSB-YP355945JAK
CSB-EP355945JAK
CSB-BP355945JAK
CSB-MP355945JAK
CSB-EP355945JAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant JC polyomavirus Major capsid protein VP1 CSB-YP355946JAK
CSB-EP355946JAK
CSB-BP355946JAK
CSB-MP355946JAK
CSB-EP355946JAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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