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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kluyveromyces lactis Protease KEX1 (KEX1), partial CSB-YP357605KBK1
CSB-EP357605KBK1
CSB-BP357605KBK1
CSB-MP357605KBK1
CSB-EP357605KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Superoxide dismutase [Mn] 3.1, mitochondrial (SODA.4) CSB-YP357606ZAX
CSB-EP357606ZAX
CSB-BP357606ZAX
CSB-MP357606ZAX
CSB-EP357606ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Interferon alpha-9 (Ifna9) CSB-YP357607MO
CSB-EP357607MO
CSB-BP357607MO
CSB-MP357607MO
CSB-EP357607MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Tubulin beta-7 chain CSB-YP357608CH
CSB-EP357608CH
CSB-BP357608CH
CSB-MP357608CH
CSB-EP357608CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Major DNA-binding protein (DBP), partial CSB-YP357609VAP
CSB-EP357609VAP
CSB-BP357609VAP
CSB-MP357609VAP
CSB-EP357609VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus DNA polymerase catalytic subunit (ORF28), partial CSB-YP357610VAP
CSB-EP357610VAP
CSB-BP357610VAP
CSB-MP357610VAP
CSB-EP357610VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Envelope glycoprotein C (gC), partial CSB-YP357612VAP
CSB-EP357612VAP
CSB-BP357612VAP
CSB-MP357612VAP
CSB-EP357612VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Envelope glycoprotein I (gI), partial CSB-YP357613VAP1
CSB-EP357613VAP1
CSB-BP357613VAP1
CSB-MP357613VAP1
CSB-EP357613VAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Envelope glycoprotein H (gH), partial CSB-YP357614VAP
CSB-BP357614VAP
CSB-MP357614VAP
CSB-EP357614VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Tegument protein UL47 homolog (ORF11), partial CSB-YP357615VAP
CSB-BP357615VAP
CSB-MP357615VAP
CSB-EP357615VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Tegument protein VP16 homolog (ORF10) CSB-YP357616VAP
CSB-EP357616VAP
CSB-BP357616VAP
CSB-MP357616VAP
CSB-EP357616VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Membrane protein 2 (ORF2) CSB-YP357617VAP
CSB-EP357617VAP
CSB-BP357617VAP
CSB-MP357617VAP
CSB-EP357617VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Transcriptional transactivator IE4 (ORF4) CSB-YP357618VAP
CSB-EP357618VAP
CSB-BP357618VAP
CSB-MP357618VAP
CSB-EP357618VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Tegument protein 7 (ORF7) CSB-YP357619VAP
CSB-EP357619VAP
CSB-BP357619VAP
CSB-MP357619VAP
CSB-EP357619VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Membrane protein UL43 homolog (ORF15), partial CSB-YP357620VAP
CSB-EP357620VAP
CSB-BP357620VAP
CSB-MP357620VAP
CSB-EP357620VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Virion host shutoff protein (17) CSB-YP357621VAP
CSB-EP357621VAP
CSB-BP357621VAP
CSB-MP357621VAP
CSB-EP357621VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Capsid assembly protein 21 (21), partial CSB-YP357622VAP
CSB-EP357622VAP
CSB-BP357622VAP
CSB-MP357622VAP
CSB-EP357622VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Capsid protein VP26 (23) CSB-YP357623VAP
CSB-EP357623VAP
CSB-BP357623VAP
CSB-MP357623VAP
CSB-EP357623VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Tripartite terminase subunit UL33 homolog (25) CSB-YP357624VAP
CSB-EP357624VAP
CSB-BP357624VAP
CSB-MP357624VAP
CSB-EP357624VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Virion egress protein 27 (27) CSB-YP357625VAP
CSB-EP357625VAP
CSB-BP357625VAP
CSB-MP357625VAP
CSB-EP357625VAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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