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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae Glycerol uptake facilitator protein (glpF), partial CSB-YP358405SMP1
CSB-EP358405SMP1
CSB-BP358405SMP1
CSB-MP358405SMP1
CSB-EP358405SMP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Peptide methionine sulfoxide reductase MsrA/MsrB 1 (msrAB1) CSB-YP358406SMP
CSB-EP358406SMP
CSB-BP358406SMP
CSB-MP358406SMP
CSB-EP358406SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae DNA mismatch repair protein hexB (hexB), partial CSB-YP358407SMP
CSB-EP358407SMP
CSB-BP358407SMP
CSB-MP358407SMP
CSB-EP358407SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae DNA mismatch repair protein HexA (hexA), partial CSB-YP358408SMP
CSB-EP358408SMP
CSB-BP358408SMP
CSB-MP358408SMP
CSB-EP358408SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pertussis toxin subunit 4 (ptxD) CSB-YP358409BIK
CSB-EP358409BIK
CSB-BP358409BIK
CSB-MP358409BIK
CSB-EP358409BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell volume regulation protein A (cvrA), partial CSB-YP358410EOD
CSB-EP358410EOD
CSB-BP358410EOD
CSB-MP358410EOD
CSB-EP358410EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neocarzinostatin (ncsA) CSB-YP358411SOV
CSB-EP358411SOV
CSB-BP358411SOV
CSB-MP358411SOV
CSB-EP358411SOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein (nifH) CSB-YP358412AJJ
CSB-EP358412AJJ
CSB-BP358412AJJ
CSB-MP358412AJJ
CSB-EP358412AJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae DNA-entry nuclease (endA), partial CSB-YP358413SMP
CSB-EP358413SMP
CSB-BP358413SMP
CSB-MP358413SMP
CSB-EP358413SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Outer membrane lipoprotein Omp16 (omp16) CSB-YP358414BMQ
CSB-EP358414BMQ
CSB-BP358414BMQ
CSB-MP358414BMQ
CSB-EP358414BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein 6b (6b) CSB-YP358415AFA
CSB-EP358415AFA
CSB-BP358415AFA
CSB-MP358415AFA
CSB-EP358415AFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 31 kDa immunogenic protein (bcsP31) CSB-YP358416BMO
CSB-BP358416BMO
CSB-MP358416BMO
CSB-EP358416BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein dpnD (dpnD) CSB-YP358417FMW
CSB-EP358417FMW
CSB-BP358417FMW
CSB-MP358417FMW
CSB-EP358417FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable spore germination protein gerPB (gerPB) CSB-YP358418BAM
CSB-EP358418BAM
CSB-BP358418BAM
CSB-MP358418BAM
CSB-EP358418BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable spore germination protein gerPD (gerPD) CSB-YP358419BAM
CSB-EP358419BAM
CSB-BP358419BAM
CSB-MP358419BAM
CSB-EP358419BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Group II intron-encoded protein ltrA (ltrA), partial CSB-YP358420LNF
CSB-EP358420LNF
CSB-BP358420LNF
CSB-MP358420LNF
CSB-EP358420LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nifZ (nifZ) CSB-YP358421KBG
CSB-EP358421KBG
CSB-BP358421KBG
CSB-MP358421KBG
CSB-EP358421KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 31 kDa outer-membrane immunogenic protein (omp31) CSB-YP358422BMO
CSB-EP358422BMO
CSB-BP358422BMO
CSB-MP358422BMO
CSB-EP358422BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein Atu3128 (Atu3128) CSB-YP358423AYS
CSB-EP358423AYS
CSB-BP358423AYS
CSB-MP358423AYS
CSB-EP358423AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 26 kDa periplasmic immunogenic protein (bp26) CSB-YP358424BMO
CSB-BP358424BMO
CSB-MP358424BMO
CSB-EP358424BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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