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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP358774MVH
CSB-EP358774MVH
CSB-BP358774MVH
CSB-MP358774MVH
CSB-EP358774MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized NTE family protein Mb2594 (Mb2594), partial CSB-YP358775MVH
CSB-EP358775MVH
CSB-BP358775MVH
CSB-MP358775MVH
CSB-EP358775MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP358776MVH
CSB-EP358776MVH
CSB-BP358776MVH
CSB-MP358776MVH
CSB-EP358776MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfur carrier protein CysO (cysO) CSB-YP358777MVH
CSB-EP358777MVH
CSB-BP358777MVH
CSB-MP358777MVH
CSB-EP358777MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv3095/MT3179 (Rv3095, MT3179) CSB-YP358778MVZ
CSB-EP358778MVZ
CSB-BP358778MVZ
CSB-MP358778MVZ
CSB-EP358778MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative diacyglycerol O-acyltransferase Mb3154c (Mb3154c) CSB-YP358779MVH
CSB-EP358779MVH
CSB-BP358779MVH
CSB-MP358779MVH
CSB-EP358779MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable VapC ribonuclease Mb2004c (Mb2004c) CSB-YP358780MVH
CSB-EP358780MVH
CSB-BP358780MVH
CSB-MP358780MVH
CSB-EP358780MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein Mb3743c (Mb3743c) CSB-YP358781MVH
CSB-EP358781MVH
CSB-BP358781MVH
CSB-MP358781MVH
CSB-EP358781MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0135 protein Rv2230c/MT2289 (Rv2230c, MT2289) CSB-YP358782MVZ
CSB-EP358782MVZ
CSB-BP358782MVZ
CSB-MP358782MVZ
CSB-EP358782MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil phosphoribosyltransferase (upp) CSB-YP358783MVZ
CSB-EP358783MVZ
CSB-BP358783MVZ
CSB-MP358783MVZ
CSB-EP358783MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit alpha (ureC), partial CSB-YP358784MVZ
CSB-EP358784MVZ
CSB-BP358784MVZ
CSB-MP358784MVZ
CSB-EP358784MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Urease subunit beta (ureB) CSB-YP358785MVZ
CSB-EP358785MVZ
CSB-BP358785MVZ
CSB-MP358785MVZ
CSB-EP358785MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease accessory protein UreG (ureG) CSB-YP358786MVZ
CSB-EP358786MVZ
CSB-BP358786MVZ
CSB-MP358786MVZ
CSB-EP358786MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative regulator of ribonuclease activity (Rv3853, MT3968) CSB-YP358787MVZ
CSB-EP358787MVZ
CSB-BP358787MVZ
CSB-MP358787MVZ
CSB-EP358787MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Immunogenic protein MPT70 (mpt70) CSB-YP358788MVZ
CSB-EP358788MVZ
CSB-BP358788MVZ
CSB-MP358788MVZ
CSB-EP358788MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-dependent repressor IdeR (ideR) CSB-YP358789MVZ
CSB-EP358789MVZ
CSB-BP358789MVZ
CSB-MP358789MVZ
CSB-EP358789MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit gamma (ureA) CSB-YP358791MVZ
CSB-EP358791MVZ
CSB-BP358791MVZ
CSB-MP358791MVZ
CSB-EP358791MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP358792MVZ
CSB-EP358792MVZ
CSB-BP358792MVZ
CSB-MP358792MVZ
CSB-EP358792MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP358793MVZ
CSB-EP358793MVZ
CSB-BP358793MVZ
CSB-MP358793MVZ
CSB-EP358793MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PE family protein PE15 (PE15) CSB-YP358794MVZ
CSB-EP358794MVZ
CSB-BP358794MVZ
CSB-MP358794MVZ
CSB-EP358794MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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