Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP359057EOD
CSB-EP359057EOD
CSB-BP359057EOD
CSB-MP359057EOD
CSB-EP359057EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP359058SWW
CSB-EP359058SWW
CSB-BP359058SWW
CSB-MP359058SWW
CSB-EP359058SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP359059ENV
CSB-EP359059ENV
CSB-BP359059ENV
CSB-MP359059ENV
CSB-EP359059ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP359060SXB
CSB-EP359060SXB
CSB-BP359060SXB
CSB-MP359060SXB
CSB-EP359060SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP359061SWW
CSB-EP359061SWW
CSB-BP359061SWW
CSB-MP359061SWW
CSB-EP359061SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP359062ENV
CSB-BP359062ENV
CSB-MP359062ENV
CSB-EP359062ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP359063EOD
CSB-EP359063EOD
CSB-BP359063EOD
CSB-MP359063EOD
CSB-EP359063EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP359064EOD
CSB-EP359064EOD
CSB-BP359064EOD
CSB-MP359064EOD
CSB-EP359064EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP359065EGX
CSB-EP359065EGX
CSB-BP359065EGX
CSB-MP359065EGX
CSB-EP359065EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli S-adenosylmethionine synthase (metK) CSB-YP359066ENV
CSB-EP359066ENV
CSB-BP359066ENV
CSB-MP359066ENV
CSB-EP359066ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP359067EOD
CSB-EP359067EOD
CSB-BP359067EOD
CSB-MP359067EOD
CSB-EP359067EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-seryl-tRNA (Sec) selenium transferase CSB-YP359068ENV
CSB-EP359068ENV
CSB-BP359068ENV
CSB-MP359068ENV
CSB-EP359068ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sugar fermentation stimulation protein A (sfsA) CSB-YP359069ENV
CSB-EP359069ENV
CSB-BP359069ENV
CSB-MP359069ENV
CSB-EP359069ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein smg (smg) CSB-YP359070SZB
CSB-EP359070SZB
CSB-BP359070SZB
CSB-MP359070SZB
CSB-EP359070SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aerobic C4-dicarboxylate transport protein (dctA), partial CSB-YP359071EGX
CSB-EP359071EGX
CSB-BP359071EGX
CSB-MP359071EGX
CSB-EP359071EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP359072EGX
CSB-EP359072EGX
CSB-BP359072EGX
CSB-MP359072EGX
CSB-EP359072EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP359073SZB
CSB-EP359073SZB
CSB-BP359073SZB
CSB-MP359073SZB
CSB-EP359073SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP359076EGX
CSB-EP359076EGX
CSB-BP359076EGX
CSB-MP359076EGX
CSB-EP359076EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP359077SZB
CSB-EP359077SZB
CSB-BP359077SZB
CSB-MP359077SZB
CSB-EP359077SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trigger factor (tig) CSB-YP359078ENV
CSB-EP359078ENV
CSB-BP359078ENV
CSB-MP359078ENV
CSB-EP359078ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>