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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Inner membrane ABC transporter permease protein ydcV (ydcV), partial CSB-YP360312SZB1
CSB-EP360312SZB1
CSB-BP360312SZB1
CSB-MP360312SZB1
CSB-EP360312SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autoinducer 2 import system permease protein lsrD (lsrD), partial CSB-YP360313SZB
CSB-EP360313SZB
CSB-BP360313SZB
CSB-MP360313SZB
CSB-EP360313SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant AI-2 transport protein tqsA (tqsA), partial CSB-YP360314SZB
CSB-EP360314SZB
CSB-BP360314SZB
CSB-MP360314SZB
CSB-EP360314SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0118 inner membrane protein ydiK (ydiK), partial CSB-YP360315SZB
CSB-EP360315SZB
CSB-BP360315SZB
CSB-MP360315SZB
CSB-EP360315SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized metalloprotease yebA (yebA), partial CSB-YP360316EGX1
CSB-EP360316EGX1
CSB-BP360316EGX1
CSB-MP360316EGX1
CSB-EP360316EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane amino-acid ABC transporter permease protein yecS (yecS), partial CSB-YP360317ENV1
CSB-EP360317ENV1
CSB-BP360317ENV1
CSB-MP360317ENV1
CSB-EP360317ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inner membrane amino-acid ABC transporter permease protein yecS (yecS), partial CSB-YP360318SZB1
CSB-EP360318SZB1
CSB-BP360318SZB1
CSB-MP360318SZB1
CSB-EP360318SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein YehT (yehT) CSB-YP360319EGX
CSB-EP360319EGX
CSB-BP360319EGX
CSB-MP360319EGX
CSB-EP360319EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0153 protein yeiW (yeiW) CSB-YP360320ENV
CSB-EP360320ENV
CSB-BP360320ENV
CSB-MP360320ENV
CSB-EP360320ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane ABC transporter permease protein yejB (yejB), partial CSB-YP360321ENV1
CSB-EP360321ENV1
CSB-BP360321ENV1
CSB-MP360321ENV1
CSB-EP360321ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized transporter YfbS (yfbS), partial CSB-YP360322ENV
CSB-EP360322ENV
CSB-BP360322ENV
CSB-MP360322ENV
CSB-EP360322ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional regulatory protein GlrR (glrR) CSB-YP360323ENV
CSB-EP360323ENV
CSB-BP360323ENV
CSB-MP360323ENV
CSB-EP360323ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yiiF (yiiF) CSB-YP360324ENV
CSB-EP360324ENV
CSB-BP360324ENV
CSB-MP360324ENV
CSB-EP360324ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Riboflavin synthase alpha chain (ribE) CSB-YP360325ENV
CSB-EP360325ENV
CSB-BP360325ENV
CSB-MP360325ENV
CSB-EP360325ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein yibH (yibH), partial CSB-YP360326ENV1
CSB-EP360326ENV1
CSB-BP360326ENV1
CSB-MP360326ENV1
CSB-EP360326ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YhiD (yhiD), partial CSB-YP360327ENV1
CSB-EP360327ENV1
CSB-BP360327ENV1
CSB-MP360327ENV1
CSB-EP360327ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable endopeptidase Spr (spr) CSB-YP360328ENV
CSB-EP360328ENV
CSB-BP360328ENV
CSB-MP360328ENV
CSB-EP360328ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable endopeptidase Spr (spr) CSB-YP360329EOD
CSB-EP360329EOD
CSB-BP360329EOD
CSB-MP360329EOD
CSB-EP360329EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phage shock protein D (pspD) CSB-YP360330ENV
CSB-EP360330ENV
CSB-BP360330ENV
CSB-MP360330ENV
CSB-EP360330ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional activator rfaH (rfaH) CSB-YP360331EOD
CSB-EP360331EOD
CSB-BP360331EOD
CSB-MP360331EOD
CSB-EP360331EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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