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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP363935ENV
CSB-EP363935ENV
CSB-BP363935ENV
CSB-MP363935ENV
CSB-EP363935ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase (prs) CSB-YP363936EGX
CSB-EP363936EGX
CSB-BP363936EGX
CSB-MP363936EGX
CSB-EP363936EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP363937ENV
CSB-EP363937ENV
CSB-BP363937ENV
CSB-MP363937ENV
CSB-EP363937ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP363938SZB
CSB-EP363938SZB
CSB-BP363938SZB
CSB-MP363938SZB
CSB-EP363938SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP363939EGX
CSB-EP363939EGX
CSB-BP363939EGX
CSB-MP363939EGX
CSB-EP363939EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP363940SZB
CSB-EP363940SZB
CSB-BP363940SZB
CSB-MP363940SZB
CSB-EP363940SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maf-like protein yceF (yceF) CSB-YP363941SZB
CSB-EP363941SZB
CSB-BP363941SZB
CSB-MP363941SZB
CSB-EP363941SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Methylglyoxal synthase (mgsA) CSB-YP363942ENV
CSB-EP363942ENV
CSB-BP363942ENV
CSB-MP363942ENV
CSB-EP363942ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylglyoxal synthase (mgsA) CSB-YP363943SZB
CSB-EP363943SZB
CSB-BP363943SZB
CSB-MP363943SZB
CSB-EP363943SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division topological specificity factor (minE) CSB-YP363944EGX
CSB-EP363944EGX
CSB-BP363944EGX
CSB-MP363944EGX
CSB-EP363944EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division topological specificity factor (minE) CSB-YP363945SZB
CSB-EP363945SZB
CSB-BP363945SZB
CSB-MP363945SZB
CSB-EP363945SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP363946EGX
CSB-EP363946EGX
CSB-BP363946EGX
CSB-MP363946EGX
CSB-EP363946EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP363947SZB
CSB-EP363947SZB
CSB-BP363947SZB
CSB-MP363947SZB
CSB-EP363947SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP363949SZB
CSB-EP363949SZB
CSB-BP363949SZB
CSB-MP363949SZB
CSB-EP363949SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP363950EGX
CSB-EP363950EGX
CSB-BP363950EGX
CSB-MP363950EGX
CSB-EP363950EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP363951ENV
CSB-EP363951ENV
CSB-BP363951ENV
CSB-MP363951ENV
CSB-EP363951ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP363952SZB
CSB-EP363952SZB
CSB-BP363952SZB
CSB-MP363952SZB
CSB-EP363952SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP363953SZB
CSB-EP363953SZB
CSB-BP363953SZB
CSB-MP363953SZB
CSB-EP363953SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP363954ENV
CSB-EP363954ENV
CSB-BP363954ENV
CSB-MP363954ENV
CSB-EP363954ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutathione-regulated potassium-efflux system ancillary protein KefG (kefG) CSB-YP363955EGX
CSB-EP363955EGX
CSB-BP363955EGX
CSB-MP363955EGX
CSB-EP363955EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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