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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Glutamate--cysteine ligase (gshA) CSB-YP363915ENV
CSB-BP363915ENV
CSB-MP363915ENV
CSB-EP363915ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamyl-tRNA reductase (hemA) CSB-YP363916ENV
CSB-EP363916ENV
CSB-BP363916ENV
CSB-MP363916ENV
CSB-EP363916ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli RNA-binding protein Hfq (hfq) CSB-YP363917ENV
CSB-BP363917ENV
CSB-MP363917ENV
CSB-EP363917ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein hfq (hfq) CSB-YP363918SZB
CSB-EP363918SZB
CSB-BP363918SZB
CSB-MP363918SZB
CSB-EP363918SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit alpha (ihfA) CSB-YP363919EGX
CSB-EP363919EGX
CSB-BP363919EGX
CSB-MP363919EGX
CSB-EP363919EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit alpha (ihfA) CSB-YP363920EOD
CSB-EP363920EOD
CSB-BP363920EOD
CSB-MP363920EOD
CSB-EP363920EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Integration host factor subunit beta (ihfB) CSB-YP363921ENV
CSB-EP363921ENV
CSB-BP363921ENV
CSB-MP363921ENV
CSB-EP363921ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit beta (ihfB) CSB-YP363922SZB
CSB-EP363922SZB
CSB-BP363922SZB
CSB-MP363922SZB
CSB-EP363922SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 33 kDa chaperonin (hslO) CSB-YP363923ENV
CSB-EP363923ENV
CSB-BP363923ENV
CSB-MP363923ENV
CSB-EP363923ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein DnaK (dnaK) CSB-YP363924ENV
CSB-EP363924ENV
CSB-BP363924ENV
CSB-MP363924ENV
CSB-EP363924ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein DnaK (dnaK) CSB-YP363925EOD
CSB-EP363925EOD
CSB-BP363925EOD
CSB-MP363925EOD
CSB-EP363925EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein hscA (hscA) CSB-YP363926EOD
CSB-EP363926EOD
CSB-BP363926EOD
CSB-MP363926EOD
CSB-EP363926EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG) CSB-YP363927EGX
CSB-EP363927EGX
CSB-BP363927EGX
CSB-MP363927EGX
CSB-EP363927EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nickel-responsive regulator (nikR) CSB-YP363928ENV
CSB-EP363928ENV
CSB-BP363928ENV
CSB-MP363928ENV
CSB-EP363928ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel-responsive regulator (nikR) CSB-YP363929EOD
CSB-EP363929EOD
CSB-BP363929EOD
CSB-MP363929EOD
CSB-EP363929EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein HypA (hypA) CSB-YP363930ENV
CSB-EP363930ENV
CSB-BP363930ENV
CSB-MP363930ENV
CSB-EP363930ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein HypA (hypA) CSB-YP363931SZB
CSB-EP363931SZB
CSB-BP363931SZB
CSB-MP363931SZB
CSB-EP363931SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable hydrogenase nickel incorporation protein HybF (hybF) CSB-YP363932SZB
CSB-EP363932SZB
CSB-BP363932SZB
CSB-MP363932SZB
CSB-EP363932SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable intracellular septation protein A (yciB), partial CSB-YP363933EOD1
CSB-EP363933EOD1
CSB-BP363933EOD1
CSB-MP363933EOD1
CSB-EP363933EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP363934EGX
CSB-EP363934EGX
CSB-BP363934EGX
CSB-MP363934EGX
CSB-EP363934EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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